This paper investigates Zn2+ and Cu2+ adsorption capability of bentonite and zeolite taken from the non-raw metallic deposits of Slovakia. Viable biomass of an Bacillus pumilus and Bacillus megaterium enhanced the efficiency of Zn2+ and Cu2+ adsorption from model solution. Initial concentration of Cu and Zn in model solutions initially containing 32.3 mg.Cu.L-1and 42.9 mg Zn.L-1 after six hours sorption and desorption at 25°C, it was observed that 1g bentonite whit bacteria inokulum was found to remove 0.195 mg Zn2+ and 0.17 mg Cu2+ from the solution and 1g zeolite was found to remove 0.088 Zn2+ and 0.051 Cu2+. The ability for Zn and Cu sorption was bentonite > zeolite. The adsorption of metal ions on bentonite and zeolite depends on pH. Between pH 4 and 6, the main mechanism is by ion exchange. In order to prevent contamination of subsoil and groundwater by leachates containing heavy metals, bentonite and zeolite are widely used as cost-effective treatments barriers. For this reason it is important to study the adsorption of metals by these materials.