Regulation of PCE-Dechlorinating Activity by Electrochemical Cultivation


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Tetrachloroethene (PCE) is one of the grave environmental pollutants. Certain anaerobic bacteria have the ability to dechlorinate PCE as an electron acceptor for their respiration (dehalorespiration). Previously, we accumulated PCE-dechlorinating culture in the presence of Fe(III)-EDTA. In this study, we have described the regulation of dehalorespiration by electrolytic control of the Fe(II)/Fe(III) ratio using a potentiostat (electrochemical cultivation). PCE-dechlorinating culture was cultivated under H2 conditions at constant redox potentials ranging from –0.1 to –0.8 V. After electrochemical cultivation for 3 weeks, PCE-dechlorinating activity of the mixed culture varied at different redox potentials. At –0.6 V, the culture showed the highest activity and the highest ratio of Desulfitobacterium/total bacteria. Electrochemical cultivation is a useful method for regulating PCE-dechlorinating bacterium.



Advanced Materials Research (Volumes 20-21)

Edited by:

Axel Schippers, Wolfgang Sand, Franz Glombitza and Sabine Willscher




S. Hirano et al., "Regulation of PCE-Dechlorinating Activity by Electrochemical Cultivation", Advanced Materials Research, Vols. 20-21, pp. 319-322, 2007

Online since:

July 2007




[1] N. Ohmura et. al: Appl. Environ. Microbiol. Vol. 68 (2002), p.405.

[2] W.F. Roling et. al: Appl. Environ. Microbiol. Vol. 68 (2002), p.5537.

[3] T.H. Smits et. al: J. Microbiol. Methods Vol. 57 (2004), p.369.

[4] S. Rozen, H. Skaletsky: Methods Mol. Biol. Vol. 132 (2000), p.365.