Effect of LPS Removal on Electrophoretic Softness of Acidithiobacillus ferrooxidans Cells


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Applicability of Ohshima’s soft-particle model to evaluate the surface potential of Acidithiobacillus ferrooxidans cells is discussed here. The electrokinetic properties were examined by electrophoretic mobility measurements and analyzed using the soft particle electrophoresis theory. As the ionic concentration increased, the mobility of the bacterial cells converged to nonzero values suggesting that the particles exhibited typical soft-particle characteristics. Also, cell surface potentials based on soft-particle theory were lower than those estimated using the conventional Smoluchowski theory. Effect of removal of LPS from the cell surface on surface softness and charge density are investigated and discussed.



Advanced Materials Research (Volumes 20-21)

Edited by:

Axel Schippers, Wolfgang Sand, Franz Glombitza and Sabine Willscher




M.N. Chandraprabha et al., "Effect of LPS Removal on Electrophoretic Softness of Acidithiobacillus ferrooxidans Cells", Advanced Materials Research, Vols. 20-21, pp. 341-344, 2007

Online since:

July 2007




[1] Ohshima, H., J. Colloid Interface Sci. 163 (1994) 474-483.

[2] Silverman, M.P. and Lundgren, D.G., J. Bacteriol. 77 (1959) 642-646.

[3] Karavaiko, G.I., In: Biotechnology of Metals - Manual, Center for International Projects GKNT, G. I. Karavaiko, G. Rossi, A. D. Agate, S. N. Groudev and Z. A. Avakyan (Eds. ), Moscow, 1988, pp.47-79.

[4] Leive, L., J. Biol. Chem. 243 (1968) 2373-2380.

[5] Leive, L., Shovlin, V.K. and Mergenhagen, S.E., J. Biol Chem. 243 (1968) 6384-6391.

[6] Tsuneda, S., Aikawa, H., Hayashi, H. and Hirata, A., J. Colloids Interface Sci. 279 (2004) 410-417.

[7] Hayashi, H., Seiki, H., Tsuneda, S., Hirata, A. and Sasaki, H., J. Colloid Interface Sc. 264 (2003) 565-568.

[8] Kiers, R.J.M., Bos, R., van der Mei, H.C. and Busscher, H.J., Microbiol. 147 (2001) 757-762.

[9] Hayashi, H., Tsuneda, S., Hirata, A. and Sasaki, H., Colloids Surfaces B: Biointerfaces. 22 (2001) 149-157.

[10] Kerchove, A.J. and Elimelech, M., Langmuir, 21 (2005) 6462-6472.

[11] van der Mei, H.C., Weerkamp, A.H. and Busscher, H.J. FEMS Microbiol. Lett. 40 (1987) 15.

[19] [12] Bos, R., Van der Mei, H.C. and Busscher, H J., Biophys. Chem. 74 (1998) 251-255.

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