Analysis of the Microbial Community in the Leachate Collected from an Experimental Bioleaching Column by Cloning and RFLP
A culture independent molecular methodology was used to investigate the bacterial and archaeal microbial dynamics of leachate collected from a 60°C chalcopyrite bioleaching column inoculated with a known microbial consortium. A 16S rRNA gene clone library was constructed for both the bacterial and archaeal populations in the leachate from the column. PCR-RFLP analysis of these clone libraries indicate species dominance and generally low species diversity.
Axel Schippers, Wolfgang Sand, Franz Glombitza and Sabine Willscher
L.A. Mutch et al., "Analysis of the Microbial Community in the Leachate Collected from an Experimental Bioleaching Column by Cloning and RFLP", Advanced Materials Research, Vols. 20-21, pp. 485-488, 2007