Enrichment of Chromium-Reducing Bacteria Using Potential-Controlled Electrochemical Cultivation


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We present here a method for the electrochemical enrichment of chromium-reducing bacteria from environmental samples containing various microorganisms. An electrochemical cultivation system was constructed; this consisted of a Pt anode and a carbon cathode separated by an ion-exchange membrane. For an electrochemical cultivation, an environmental sample with chromium-reducing bacteria was inoculated into the anode well where a medium containing 0.1 mM Cr(VI) ions was poured. On the other hand, Cr(VI)-free medium was poured into the cathode well. Chromium-reducing bacteria grew selectively after 10 days of incubation and application of 1.0 V (vs. Ag/AgCl) of anode potential that reproduces Cr(VI) ions. This result suggested that Cr(VI) ions generated on the anode promoted the growth of chromium-reducing bacteria as their electron acceptor and due to Cr(VI) toxicity, inhibited the growth of other microorganisms.



Advanced Materials Research (Volumes 20-21)

Edited by:

Axel Schippers, Wolfgang Sand, Franz Glombitza and Sabine Willscher




N. Matsumoto et al., "Enrichment of Chromium-Reducing Bacteria Using Potential-Controlled Electrochemical Cultivation", Advanced Materials Research, Vols. 20-21, pp. 505-508, 2007

Online since:

July 2007




[1] L. Diels et al.: Mol. Biotechnol. Vol. 12 (1999), p.149.

[2] A. Ganguli and A.K. Tripathi: Appl. Microbiol. Biotechnol. Vol. 58 (2002), p.416.

[3] N. Matsumoto et al.: Biotechnol. Bioeng. Vol. 64 (1999), p.716.

[4] N. Ohmura et al.: Appl. Environ. Microbiol. Vol. 68 (2002), p.405.