Rapid Preparation of Pure Antibodies against Classical Swine Fever Virus from Pig Serum by Immunoaffinity Chromatography

Abstract:

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After Sepharose-4B polymerbeads were activated by using epichlorohydrin, purified swine fever virus as a ligand were binded with them to prepare an immobilized affinity chromatography column, which was used to prepare antibody from high immune pig serum. Equilibrated with pH 7.4, 0.02 mol/L PBS and eluated with pH 3.4, 0.2 mol/L NaAc–HAc buffer containing 0.5 mol/L NaCl, the purified protein obtained from this columne was demonstrated to have normal activity to combine with classical swine fever virus by SDS-PAGE and ELISA. The extraction efficiency of the antibody was 2.45% of total proteins. This study offers a novel, rapid and effective method for preparation of pure antibodies against classical swine fever virus from high immune pig serum.

Info:

Periodical:

Advanced Materials Research (Volumes 201-203)

Edited by:

Daoguo Yang, Tianlong Gu, Huaiying Zhou, Jianmin Zeng and Zhengyi Jiang

Pages:

691-695

DOI:

10.4028/www.scientific.net/AMR.201-203.691

Citation:

G. Z. Wang et al., "Rapid Preparation of Pure Antibodies against Classical Swine Fever Virus from Pig Serum by Immunoaffinity Chromatography", Advanced Materials Research, Vols. 201-203, pp. 691-695, 2011

Online since:

February 2011

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Price:

$35.00

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