Rapid Preparation of Pure Antibodies against Classical Swine Fever Virus from Pig Serum by Immunoaffinity Chromatography
After Sepharose-4B polymerbeads were activated by using epichlorohydrin, purified swine fever virus as a ligand were binded with them to prepare an immobilized afﬁnity chromatography column, which was used to prepare antibody from high immune pig serum. Equilibrated with pH 7.4, 0.02 mol/L PBS and eluated with pH 3.4, 0.2 mol/L NaAc–HAc buffer containing 0.5 mol/L NaCl, the purified protein obtained from this columne was demonstrated to have normal activity to combine with classical swine fever virus by SDS-PAGE and ELISA. The extraction efficiency of the antibody was 2.45% of total proteins. This study offers a novel, rapid and effective method for preparation of pure antibodies against classical swine fever virus from high immune pig serum.
Daoguo Yang, Tianlong Gu, Huaiying Zhou, Jianmin Zeng and Zhengyi Jiang
G. Z. Wang et al., "Rapid Preparation of Pure Antibodies against Classical Swine Fever Virus from Pig Serum by Immunoaffinity Chromatography", Advanced Materials Research, Vols. 201-203, pp. 691-695, 2011