Molecular Cloning and Phylogenetic Analysis of the Duck Enteritis Virus UL55 Gene


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A 561-bp complete open reading frame of the duck enteritis virus(DEV) UL55 gene (GenBank accession No EU071034) was isolated in our laboratory and amplified by common PCR using a pair of specific primers .PCR product containing this ORF was cloned into the vector of PMD18-T. Four evolutionary analysis approaches were used to construct phylogenetic trees of DEV in relation to herpesviruses based on the nucleotide sequences. Bootstrap was used as statistic method to testify the reliablity of the constructed phylogenetic tree. Phylogenetic trees indicated that DEV and other herpesviruses generated from one ancestor and DEV were grouped into the subfamily Alphaherpesvirinae. In our result, DEV showed a close relationship with the genus Mardivirus, but formed a single branch. Partial genomic organization and phylogenetic analysis in the present study provides evidence that DEV was a member of the subfamily Alphaherpesvirinae and should be assigned as an individual genus or group.



Advanced Materials Research (Volumes 204-210)

Edited by:

Helen Zhang, Gang Shen and David Jin






Y. Wu et al., "Molecular Cloning and Phylogenetic Analysis of the Duck Enteritis Virus UL55 Gene", Advanced Materials Research, Vols. 204-210, pp. 663-671, 2011

Online since:

February 2011




[1] S. Davison, K. Converse, A. Hamir, and R. Eckroade: Avian diseases vol. 37(1993), pp.1142-1146.

[2] R. Montgomery, G. Stein Jr, M. Novilla, S. Hurley, and R. Fink: Avian diseases(1981), pp.207-213.

[3] E. Campagnolo, M. Banerjee, B. Panigrahy, and R. Jones: Avian diseases vol. 45(2001), pp.522-528.

[4] S. Shawky, and K. Schat: Journal Information vol. 46(2002).

[5] C. Fauquet, M. Mayo, J. Maniloff, U. Desselberger, and L. Ball, Virus taxonomy: eighth report of the International Committee on Taxonomy of Viruses: elsevier academic press London, (2005).

[6] DanielCEdelman: Virology Journal vol. 2(2005).

[7] L. Pomeranz, A. Reynolds, and C. Hengartner: Microbiology and molecular biology reviews vol. 69(2005), p.462.

[8] R. B, and P. PE, The family Herpesviridae: a brief introduction, In Fields virology Volume 2. 4th edition, K. DM, H. PM, P. Philadelphia, L. Williams and Wilkins, eds., pp.2381-2397, (2001).

[9] R. An, H. Li, Z. Han, Y. Shao, S. Liu, and X. Kong: Acta virologica vol. 52(2008), p.23.

[10] H. Li, S. Liu, Z. Han, Y. Shao, S. Chen, and X. Kong: Genetics and Molecular Biology vol. 32(2009), pp.121-128.

[11] H. Li, S. Liu, and X. Kong: Virus Genes vol. 33(2006), pp.221-227.

[12] S. Liu, S. Chen, H. Li, and X. Kong: Gene vol. 401(2007), pp.88-96.

[13] Y. Zhao, J. Wang, F. Liu, and B. Ma: Virus Genes vol. 38(2009), pp.243-248.

[14] Y. Zhao, J. Wang, B. Ma, and F. Liu: Virus Genes vol. 38(2009), pp.289-294.

[15] ChanjuanShen, YufeiGuo, AnchunCheng, MingshuWang, YiZhou, DanLin, HongyiXin, and NaZhang: Virology Journal vol. 6(2009).

[16] WeiXie, AnchunCheng, MingshuWang, HuaChang, DekangZhu, QihuiLuo, RenyongJia, and XiaoyueChen: Virology Journal vol. 6(2009), p.10.

[17] F. Liu, B. Ma, Y. Zhao, Y. Zhang, Y. H. Wu, X. Liu, and J. Wang: Virus Genes vol. 37(2008), p.328–332.

[18] Y. Wu, A. Cheng, and M. Wang, Molecular Characterization Analysis of Newly identified Duck Enteritis Virus UL55 Gene, in The 4th International Conference on Bioinformatics and Biomedical Engineering (iCBBE 2010) Chengdu, China, 2010, pp. inpress.

DOI: 10.1109/icbbe.2010.5515663

[19] P. Plummer, T. Alefantis, S. Kaplan, P. O'Connell, S. Shawky, and K. Schat: Avian diseases vol. 42(1998), pp.554-564.

DOI: 10.2307/1592682

[20] W. Hansen, S. Brown, S. Nashold, and D. Knudson: Avian diseases(1999), pp.106-115.

[21] G. Xiao-feng, I. Ming, and X. Chao-an: Acta Veterinaria et Zootechnica Sinca vol. 33(2002), pp.615-618.

[22] Q. Zou, K. Sun, A. Cheng, M. Wang, C. Xu, D. Zhu, R. Jia, Q. Luo, Y. Zhou, Z. Chen, X. Chen, and VirologyJournal(2010).

[23] Y. Li, B. Huang, X. Ma, J. Wu, F. Li, W. Ai, M. Song, and H. Yang: Virology(2009).

[24] L. Zhao, A. Cheng, M. Wang, G. Yuan, R. Jia, D. Zhou, X. Qi, H. Ge, and T. Sun: Avian Diseases vol. 52(2008).

[25] X. R. Zhong, A. C. Cheng, M. S. Wang, Y. Q. Gong, D. K. Zhu, R. Y. Jia, and Q. H. Luo, Molecular cloning and sequence analysis of UL34 gene from duck plague virus CHv strain, in IEEE, the 2nd International Conference on BioMedical Engineering and Informatics, Tianjing, China, 2009, pp.1478-1485.

DOI: 10.1109/bmei.2009.5305673

[26] J. Xiang, A. Cheng, M. Wang, H. Chang, and W. Chen, Molecular Cloning and Sequence Analysis of the Duck Enteritis Virus Nucleocapsid Gene(UL38) , in IEEE, the 2nd International Conference on BioMedical Engineering and Informatics, Tianjing, China, 2009, pp.1874-1880.

DOI: 10.1109/bmei.2009.5305097

[27] M. Kimura: Journal of molecular evolution vol. 16(1980), pp.111-120.

[28] A. Davison, M. Benko, and B. Harrach: Journal of General Virology vol. 84(2003), p.2895.

[29] C. Gubser, S. Hue, P. Kellam, and G. Smith: Journal of General Virology vol. 85(2004), p.105.

[30] D. Bulach, C. Kumar, A. Zaia, B. Liang, and D. Tribe: Journal of invertebrate pathology vol. 73(1999), pp.59-73.

[31] X. Chen, W. IJkel, C. Dominy, P. de Andrade Zanotto, Y. Hashimoto, O. Faktor, T. Hayakawa, C. Wang, A. Prekumar, and S. Mathavan: Virus Research vol. 65(1999), pp.21-32.

DOI: 10.1016/s0168-1702(99)00097-0

[32] E. Koonin: Journal of General Virology vol. 72(1991), p.2197.

[33] U. Melcher: Journal of General Virology vol. 81(2000), p.257.

[34] F. Tetart, C. Desplats, M. Kutateladze, C. Monod, H. Ackermann, and H. Krisch: Journal of Bacteriology vol. 183(2001), p.358.

[35] C. Tidona, and G. Darai: Virus Genes vol. 21(2000), pp.77-81.

[36] C. Tidona, P. Schnitzler, R. Kehm, and G. Darai: Virus Genes vol. 16(1998), pp.59-66.

DOI: 10.1023/a:1007949710031

[37] L. Gao, and J. Qi: BMC Evolutionary Biology vol. 7(2007), p.7.

[38] M. Alb¨¤, R. Das, C. Orengo, and P. Kellam: Genome Research vol. 11(2001), p.43.

[39] D. McGeoch, A. Dolan, and A. Ralph: The Journal of Virology vol. 74(2000), p.10401.

[40] A. Richardson, and J. Palmer: Journal of experimental botany vol. 58(2007), p.1.

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