Study on Reaction Kinetics of Lipase-Catalyzed Synthesis of Vanillyl Nonanoate in Acetone Media

Abstract:

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The reaction kinetics for synthesis of vanillyl nonanoate (VN) by lipase-catalyzed transesterification of vanillyl alcohol and methyl nonanoate in acetone was investigated in this study. The reaction catalyzed by lipase was carried out as follows: A reaction mixture containing given concentration of substrates (1ml) and lipase Novozyme 435 (20mg) in acetone (1ml) was shaken at 30°C for 10min. The initial velocity of the reaction was calculated according to the concentration of VN detected by high performance liquid chromatography (HPLC), and the kinetic equation was obtained by analysis of the double reciprocal plot of initial velocity versus substrate concentration. The data calculated by the kinetic equation were basically in agreement with the experimental data with a correlation coefficient at 0.997. The inhibitory action of methanol (by-product) in the reaction was also investigated and the results indicated that the concentration of methanol influences both slope and intercept of y-axis of the double reciprocal curve, and the lines intersect on the X-axis. These results revealed that this transesterification follows the Ping-Pong reaction mechanism.

Info:

Periodical:

Advanced Materials Research (Volumes 233-235)

Edited by:

Zhong Cao, Lixian Sun, Xueqiang Cao, Yinghe He

Pages:

2660-2664

DOI:

10.4028/www.scientific.net/AMR.233-235.2660

Citation:

H. L. Liu et al., "Study on Reaction Kinetics of Lipase-Catalyzed Synthesis of Vanillyl Nonanoate in Acetone Media", Advanced Materials Research, Vols. 233-235, pp. 2660-2664, 2011

Online since:

May 2011

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Price:

$35.00

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