Effect of Microwave Irradiation on the Structure of α –Amylase
Discuss the effect of microwave irradiation and water bath on the primary and secondary structure of α –amylase. The result showed that, α -amylase’s molecular mass didn’t change under two different condition, it means that α -amylase’s primary structure didn’t change; While the peak height (at λ=193nm) of the CD spectra of the samples treated by microwave irradiation and water bath both reduced, the reduced rate by microwave irradiation ranged from 140 % to 220 %, while the reduced rate by water bath ranged from 60 % to 140 %; the peak of the sample treated by microwave irradiation for 60 min disappeared at λ=193nm, while showed a wake peak by water bath; the negative peak position by microwave irradiation emerged a blue shift in the range of 5 ~ 8 nm at λ=204 nm and λ=220 nm, while emerged a blue shift in the range of 3 ~ 5 nm by water bath. With time going, both microwave irradiation and water bath have prompted the secondary structure on α-helix, β-sheet, β-turn and random coil’s mutual transformations, but the trends were different.
Zhong Cao, Yinghe He, Lixian Sun and Xueqiang Cao
X. Y. Zhang and L. Tan, "Effect of Microwave Irradiation on the Structure of α –Amylase", Advanced Materials Research, Vols. 236-238, pp. 1023-1028, 2011