Effect of Microwave Irradiation on the Structure of α –Amylase

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Discuss the effect of microwave irradiation and water bath on the primary and secondary structure of α –amylase. The result showed that, α -amylase’s molecular mass didn’t change under two different condition, it means that α -amylase’s primary structure didn’t change; While the peak height (at λ=193nm) of the CD spectra of the samples treated by microwave irradiation and water bath both reduced, the reduced rate by microwave irradiation ranged from 140 % to 220 %, while the reduced rate by water bath ranged from 60 % to 140 %; the peak of the sample treated by microwave irradiation for 60 min disappeared at λ=193nm, while showed a wake peak by water bath; the negative peak position by microwave irradiation emerged a blue shift in the range of 5 ~ 8 nm at λ=204 nm and λ=220 nm, while emerged a blue shift in the range of 3 ~ 5 nm by water bath. With time going, both microwave irradiation and water bath have prompted the secondary structure on α-helix, β-sheet, β-turn and random coil’s mutual transformations, but the trends were different.

Info:

Periodical:

Advanced Materials Research (Volumes 236-238)

Edited by:

Zhong Cao, Yinghe He, Lixian Sun and Xueqiang Cao

Pages:

1023-1028

DOI:

10.4028/www.scientific.net/AMR.236-238.1023

Citation:

X. Y. Zhang and L. Tan, "Effect of Microwave Irradiation on the Structure of α –Amylase", Advanced Materials Research, Vols. 236-238, pp. 1023-1028, 2011

Online since:

May 2011

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Price:

$35.00

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