Rapid Purification of Anti-SARS-CoV Protein N Monoclonal Antibody by Using Magnetic Beads

Abstract:

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A rapid and simple antibody purification method is reported for the isolation of monoclonal antibody IgG1 from SARS-CoV N protein rabbit serum by using surface-modified magnetic beads. First magnetic poly (methacrylate-divinylbenzene) (PMA-DVB) beads were prepared and modified with aldehyde end group through surface chemical reactions. Then protein A were covalently bound to aldehyde-activated magnetic beads and further used to purify monoclonal antibody IgG1 from rabbit serum directly. This rapid magnetic carrier technology proves to be efficient and rapid for antibody purification and has potential to massive production of monoclonal antibody.

Info:

Periodical:

Advanced Materials Research (Volumes 236-238)

Edited by:

Zhong Cao, Yinghe He, Lixian Sun and Xueqiang Cao

Pages:

2146-2149

Citation:

Y. X. Yu and X. Q. Liu, "Rapid Purification of Anti-SARS-CoV Protein N Monoclonal Antibody by Using Magnetic Beads", Advanced Materials Research, Vols. 236-238, pp. 2146-2149, 2011

Online since:

May 2011

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$38.00

[1] W. L. Liu, Y. Lu and Y. H. Chen: submitted to Progress in Natural Sci. (2003).

[2] F. Gao, H. Y. Ou, L. L. Chen and W. X. Zheng: submitted to Febs Letters (2003).

[3] S. Q. Liu, T. Guo, X. L. Li and Z. R. Sun: submitted to Chinese Sci. Bull. (2003).

[4] L. Qin, B. Xiong and C. Luo: submitted to Acta Pharm. Sinica (2003).

[5] L. Ding, Z. Yao, T. Li, Q. Yue and J. Chai: submitted to J. Chem. (2003).

[6] G. Xie, Q. Y. Zhang and Z. P. Luo: submitted to J. Appl. Polym. Sci. (2003).

[7] L. Shen, A. Stachowiak, T. A. Hatton and P. E. Laibinis: submitted to Langmuir (2000).

[8] Y. Lee, J. Rho and B. Jung: submitted to J. Appl. Polym. Sci. (2003).

[9] Z. Guo, S. Bai and Y. Sun: submitted to Enzyme Microb. Technol. (2003).