Rapid Purification of Anti-SARS-CoV Protein N Monoclonal Antibody by Using Magnetic Beads
A rapid and simple antibody purification method is reported for the isolation of monoclonal antibody IgG1 from SARS-CoV N protein rabbit serum by using surface-modified magnetic beads. First magnetic poly (methacrylate-divinylbenzene) (PMA-DVB) beads were prepared and modified with aldehyde end group through surface chemical reactions. Then protein A were covalently bound to aldehyde-activated magnetic beads and further used to purify monoclonal antibody IgG1 from rabbit serum directly. This rapid magnetic carrier technology proves to be efficient and rapid for antibody purification and has potential to massive production of monoclonal antibody.
Zhong Cao, Yinghe He, Lixian Sun and Xueqiang Cao
Y. X. Yu and X. Q. Liu, "Rapid Purification of Anti-SARS-CoV Protein N Monoclonal Antibody by Using Magnetic Beads", Advanced Materials Research, Vols. 236-238, pp. 2146-2149, 2011