Extraction and Partial Characterization of Pepsin-Soluble Collagens from the Skin of Amiurus Nebulosus
Pepsin-soluble collagen (PSC) was successfully extracted from the skin of Amiurus nebulosus. The skin of Amiurus nebulosus was immersed in 0.3 mol/L acetic acid (1: 20, m: V) for 6 h at 37°C, while pepsin was added, at a level of 5000U/g dosage of defatted skin. The maximal yield of the collagen was 97.44%, which was higher than that of acid-soluble collagen (ASC) at 62.05%. Some properties of pepsin-soluble collagens from the skin of Amiurus nebulosus were characterized. Amino acid composition and SDS-PAGE suggested that the collagen might be classified as type I collagen. Moreover, FTIR investigations showed the existence of helical arrangements in PSC of Amiurus nebulosus skin of collagen. There is a possibility to use Amiurus nebulosus skin collagen as an alternative source of collagen for industrial purposes and subsequently it may maximize the economical value of the fish.
Zhong Cao, Yinghe He, Lixian Sun and Xueqiang Cao
L. L. Chen et al., "Extraction and Partial Characterization of Pepsin-Soluble Collagens from the Skin of Amiurus Nebulosus", Advanced Materials Research, Vols. 236-238, pp. 2926-2934, 2011