Direct Electrochemistry and Electrocatalysis of Cytochrome C on the Interface of Ionic Liquid Functionalized Nano-Gold

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A novel reagentless H2O2 biosensor based on ionic liquid 1-butyl-3-methylimidazolium hexafluorophosphate ([Bmim]PF6) functionalized nano-gold as the biocompatible interface of Cytochrome C (Cyt c) by self-assembled method was proposed. The immobilized Cyt c showed direct electrochemistry and displayed an excellent electrocatalytic response to the reduction of H2O2 without the aid of any electron mediator. Under optimal experimental conditions, the as-prepared biosensor showed a wide linear response to H2O2 in the range of 0.23 µM to 3.0 mM with a detection limit of 0.12 µM (S/N = 3). The resulting biosensor can reached 95% of steady-state current within 5 s. The Kappm value for H2O2 was found to be 1.2 mM.

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Periodical:

Advanced Materials Research (Volumes 239-242)

Edited by:

Zhong Cao, Xueqiang Cao, Lixian Sun, Yinghe He

Pages:

1875-1878

DOI:

10.4028/www.scientific.net/AMR.239-242.1875

Citation:

K. Wang et al., "Direct Electrochemistry and Electrocatalysis of Cytochrome C on the Interface of Ionic Liquid Functionalized Nano-Gold", Advanced Materials Research, Vols. 239-242, pp. 1875-1878, 2011

Online since:

May 2011

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$35.00

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