A novel amphiphlic adsorbent resin of macroporous polystyrene Validamycin A was synthesized via the amination of chloromethylated macroporous crosslinked polystyrene resin using Validamycin A. Its chemical structure was characterized by Fourier transformation infrared spectra and elemental analysis. Its pore structure was determined by nitrogen adsorption-desorption measurements. Test results of swelling ratio in various solvents revealed that the aminated resin was amphiphilic. The resin showed excellent adsorption performance for the amphiphilic organic compounds of pueraia isoflavones in aqueous solution.