This paper studies the modification of petroleum asphalt with enzymatic hydrolysis lignin (EHL) epoxy resin. EHL epoxy resin was successfully synthesized by EHL, a novel eco-material with high chemical reactivity. Different amounts of EHL epoxy resin were compounded into AH-70 paving asphalt. The effect of EHL epoxy resin on modified asphalt was examined by penetration, soften point, thin film oven test (TFOT), ductilily tests. The results showed that EHL epoxy resin had marked effect on the high-temperatrue property of increasing softening point at the content of 2-9wt% and it had significantly effect on the low-temperature properties and aging resistance with EHL epoxy resin content of 2-9wt% in modified asphalts. TG analysis indicated that EHL epoxy resin had the high mass loss temperature than pure asphalt. Anti-aging property of asphalt was improved by compounding EHL epoxy resin with asphalt.