During the tunnel excavation in Zhang-Shi Expressway 2nd-Stage Project, rock burst phenomenon has occurred. Limy dolomite obtained from tunnel rock burst area is used to conduct laboratory loading rock burst test with simulation system of deep rock burst. Simultaneously, based on Particle Flow Theory and PFC3D program, a load-unload code is developed by fish language embedded in PFC3D to carry out simulation of laboratory loading rock burst test. Laboratory test result shows that the rock burst type and intensity of specimen are lagged and severe, respectively; During the test process, a spot of particles eject from the exposed surface; Obvious slabbing phenomenon can be seen on the exposed surface and the separate face of rock slab is parallel to the direction of maximal load σ1 as consistent with the actual phenomenon of engineering rock burst. In the loading rock burst simulation, the load grade, stress-step curve and failure mode of rock burst are almost consistent with the laboratory test. This simulation method describes the failure process actually and subtly, and reveals that the specimen fracture mechanism of loading rock burst is tensile type. Finally, some differences between loading and unloading tests of rock burst are discussed.