Chroogomphis rutillus extracts were prepared with solvent of petroleum ether, acetic ester, methanol and water. The effects of four different extract on nitrite scavenging (NS) and nitrosamine formation inhibiting (NFI) were evaluated in vitro systems. Results showed that all the extract showed NS and NFI potentials with a dose-dependent manner. With regard to NS activity, at 50μg/mL, the scavenging rates of ethyl acetate and methanol extracts were 96.65% and 93.48%, respectively. While, those of petroleum ether and water extract were only 86.96% and 26.09%, respectively. With regard to NFI activity, at 200μg/mL, both petroleum ether and ethyl acetate extracts showed excellent inhibition abilities of 94.46% and 91.33%, respectively, much higher than those of methanol and water extract (78.57% and 4.06%, respectively) (P<0.05). Chroogomphis rutillus especially ethyl acetate extract may have a preventive effect against carcinogenesis induced by nitrosamines.