Recognition of Papain by Konjac Glucomannan-Based Molecularly Imprinted Membrane

Abstract:

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Objective: To prepare a novel protein molecularly imprinted membrane. Methods: Konjac glucomanan was swollen, cross-linked, added papain and glycerol, dried to form imprinted membranes. Results: After eluted, the rebinding adsorption amounts of papain for imprinted and blank membranes were (12.692±3.452) μg/cm2 and (7.849±2.321) μg/cm2 respectively. The papain imprinted membrane had the highest imprinted factor α 1.8 for papain than for other proteins. Results of FT-IR and SEM showed that the cavity structure of the eluted membrane provided space for rebinding. Conclusions: Konjac glucomannan-based imprinted membrane has good specific adsorption and selective recognition abilities for the templates- papain, and shows great possibilities to be a promising protein molecularly imprinted polymer.

Info:

Periodical:

Advanced Materials Research (Volumes 282-283)

Edited by:

Helen Zhang and David Jin

Pages:

687-690

DOI:

10.4028/www.scientific.net/AMR.282-283.687

Citation:

Z. Y. Mi et al., "Recognition of Papain by Konjac Glucomannan-Based Molecularly Imprinted Membrane", Advanced Materials Research, Vols. 282-283, pp. 687-690, 2011

Online since:

July 2011

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Price:

$35.00

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