Two-photon three-dimensional optical data storage is an effective method for high capacity data storage. Based on DVD pick-up head and two-photon absorption, two-photon three-dimensional optical disk storage system is built, and operating principle of the overall system is in detail. In the servo module of three-dimensional optical disk storage system, DVD circuit control board (MT1389SE) is conducted on three-point improvement: extract focus servo error signal and servo error signal, increase four communications interfaces，increase photoelectric detectors; the extraction of focus and track servo signal on reading and writing module is analyzed in detailed. The measured principles and methods of the voice coil motor characteristic curve of Sanyo DVD pick-up head (SF-HD60S) are analyzed in detailed by using dual-frequency laser interferometer, which can be well meet the layer selection requirements of three-dimensional optical storage. DVD pick-up head applied in three-dimensional optical storage overcomes the limitations of three-dimensional scanning stage, integrates with existing DVD servo technology, and lays a solid foundation for practical use of two-photon three-dimensional optical storage.