A design of wireless pulse signal acquisition system for status monitoring of ship navigators has been presented. The system employs STC12LE5608AD as controller for timing acquisition of the pulse signal. And then the data are written to the register of CC2500 via SPI interface and transmitted. When the receiver takes the data, the MCU reads the data from the receiver register of CC2500. Finally the MCU send the data to computer via UART for display and further processing. Wireless transmission insures that the system can analyze the degree of navigators' fatigue objectively and quantifiably without affecting their operation of the ship. The system serves as a warning for fatigue navigation. This system has advantages of low power consumption and portability. It has the practical value of safe navigation of ships.