Fish wastes such as bones and skins, has been under-utilized. Gelatin was extracted from frozen Arrowtooth flounder (Atheresthes stomias) skin (AFS), and the edible gelatin film was successfully prepared. The results showed that the proximate composition of moisture, protein, lipid and ash of the extracted gelatin was 6.0 %, 92.8 %, 0.31 % and 0.39 %, respectively. The pH, bloom strength and viscosity of gelatin was 6.0, 234.8 g and 4.3 mPa.s, respectively. The tensile strength (TS), elongation at break (EAB), solubility and the prepared edible film were 10.3 MPa, 51.4 %, 98.3 %, and the film was transparent.