Evolutionary engineering is a novel whole-genome wide engineering strategy inspired by natural evolution for strain improvement. Terephthalic acid (TA) is the most important starting material for making synthetic products, especially polyester fiber and plastics. Both TA and its manufacturing wastewater show acute, chronic and molecular toxicity to organisms. In textiles manufacturing, alkali de-weighting finishing of polyester fabrics was a main resource for producing waster water containing TA. Microorganisms can hardly adapt to the high pH caused by the alkali in wastewater of alkali de-weighting process. Although the strain for TA biodegradation already have been isolated, it is necessary to develop new innovative methods of treating TA in alkali de-weighting wastewater effectively. In this investigation, evolutionary engineering was applied to improve the characterization of Bacillus strain and the high pH resistance phenotypes were obtained. The approach of selection procedure presented in this investigation may provide an attractive promising alternative for improvement of pH resistance strain.