In this study, electromagnetic radiation (EMR) shielding fabric was coated by Carbonyl Iron and Nickel powder. X-ray diffraction (XRD) measure was applied to test the existence of Carbonyl Iron and Nickel. In order to study protective performance of EMR shielding fabric for living body, tests on biological effect protection were proceeded respectively using EMR shielding fabric, stainless steel fiber blend fabric and common fabric of the same specification. The results showed that colony forming unit-granulocyte and macrophage (CFU-GM), which expressed the ability of the mice’s hematopoietic function, were obviously improved after using shielding fabric. And all the other biological characterizations of the life that covered the EMR shielding fabric and stainless steel fiber blend fabric were better than that that covered common fabric. Moreover, The EMR shielding fabrics had better protective effect than pure stainless steel fiber blended fabric. The parameters of the Carbonyl Iron and Nickel coated and the stainless steel fiber were the significantly factors to affect the shielding property.