Significant Enhancement of Triterpenoids Production by Phomopsis Elicitors in Cell Suspension Cultures of Betula Platyphylla Suk.
58 fungal elicitors prepared from the endophytic fungi isolated from inner bark of B. platyphylla Suk. were examined to determine their effects on the growth and triterpenoid production in suspension cultures of Betula platyphylla Suk. cells. The results showed that different fungal elicitors could cause diversely stimulating effects. Among the fungal elicitors tested, BE58 stimulated the highest triterpenoids yield with 15.90 mg•L-1 and 183.72% higher than the control. The experiment also affirmed from mRNA level that triterpenoid was indeed accumulated in suspension culture of birch cells treated by BE58 fungal elicitor. Meantime BE58 was identified as Phomopsis from the morphological and molecular level.
Yun-Hae Kim, Prasad Yarlagadda, Xiaodong Zhang and Zhijiu Ai
Q. L. Zhai et al., "Significant Enhancement of Triterpenoids Production by Phomopsis Elicitors in Cell Suspension Cultures of Betula Platyphylla Suk.", Advanced Materials Research, Vols. 335-336, pp. 105-110, 2011