Dynamic Investigation of Sensitivity and Action Mechanism of Antitumor Drug
Dynamic responses of A549 cells to epirubicin (EPI), daunorubicin (DNR), cisplatin (DDP) and carboplatin (CBP) were monitored by real-time cell electronic sensing (RT-CES) system. A new defined parameter, the detachment duration, was defined to quantitatively characterize drug-cell action mechanism. For DNR, there were two different linear dependent regions of the detachment duration. The rate constant of DNR in lower concentration region (20.4±2.0 h/μM) is much larger than that in higher concentration region (3.2±0.1 h/μM). Moreover, the rate constant of DNR in lower concentration region is close to that of EPI in higher concentration region (17.5±0.5 h/μM). Similar observation occurred in the case of DDP and CBP treatment, and the rate constant is (2.8±0.4)×10 h/μM and (4.6±1.3)×10 h/μM respectively. Taken together, A549 cells are more sensitive to DNR than EPI and to DDP than CBP. This study provides new insight into cell-drug interaction. RT-CES system may play important role in future drug screening, mechanism exploration and clinical therapy.
X. Liu et al., "Dynamic Investigation of Sensitivity and Action Mechanism of Antitumor Drug", Advanced Materials Research, Vols. 343-344, pp. 476-482, 2012