Dynamic Investigation of Sensitivity and Action Mechanism of Antitumor Drug

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Dynamic responses of A549 cells to epirubicin (EPI), daunorubicin (DNR), cisplatin (DDP) and carboplatin (CBP) were monitored by real-time cell electronic sensing (RT-CES) system. A new defined parameter, the detachment duration, was defined to quantitatively characterize drug-cell action mechanism. For DNR, there were two different linear dependent regions of the detachment duration. The rate constant of DNR in lower concentration region (20.4±2.0 h/μM) is much larger than that in higher concentration region (3.2±0.1 h/μM). Moreover, the rate constant of DNR in lower concentration region is close to that of EPI in higher concentration region (17.5±0.5 h/μM). Similar observation occurred in the case of DDP and CBP treatment, and the rate constant is (2.8±0.4)×10 h/μM and (4.6±1.3)×10 h/μM respectively. Taken together, A549 cells are more sensitive to DNR than EPI and to DDP than CBP. This study provides new insight into cell-drug interaction. RT-CES system may play important role in future drug screening, mechanism exploration and clinical therapy.

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Periodical:

Advanced Materials Research (Volumes 343-344)

Edited by:

David Wang

Pages:

476-482

DOI:

10.4028/www.scientific.net/AMR.343-344.476

Citation:

X. Liu et al., "Dynamic Investigation of Sensitivity and Action Mechanism of Antitumor Drug", Advanced Materials Research, Vols. 343-344, pp. 476-482, 2012

Online since:

September 2011

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$35.00

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