Research on the Characteristics and Culture Conditions of Saccharomyces boulardii


Article Preview

The morphological, physiological and biochemical characteristics of Saccharomyces boulardii were studied for the preparation of highly active yeast. The optimal condition of the flask culture including inoculum size, culture temperature, pH value, were investigated separately. The results showed that S.boulardii was asporogenous and had better heat tolerance and acid tolerance growing well at 37°C and pH 2.0. We also found that thermal death temperature was 55~56°C and S.boulardii is well ethanol-tolerant, for the highest concentration of ethanol that S.boulardii can tolerate was 20%. The optimal culture conditions in a flask culture was YEPD medium with 50ml/250ml medium volume, natural pH, 5% inoculum size, 32°C culture temperature, 160 r/min shaker speed, and 24 hours cultivation period. Under these conditions, suspension was diluted 10 times and the optical density at 600 nm was up to 0.85.



Advanced Materials Research (Volumes 343-344)

Edited by:

David Wang




L. P. Du et al., "Research on the Characteristics and Culture Conditions of Saccharomyces boulardii ", Advanced Materials Research, Vols. 343-344, pp. 594-598, 2012

Online since:

September 2011




[1] M. Mallié, P. Nguyen Van, S. Bertout, C. Vaillant, J. -M. Bastide, Genotypic study of Saccharomyces boulardii compared to the Saccharomyces sensu stricto complex species, J. Mycol. Med. 11(2001) 19–25.

[2] D. Czerucka, and P. Rampal. 2002. Experimental effects of Saccharomyces boulardii on diarrheal pathogens. Microbes Infect. 4: 733–739.


[3] Jie Chu, Fengshan Wang. Research progress on the biological and therapeutic effects of S. boulardii [J]. Pharmaceutical Biotechnology, 2006 , 13(1): 71-73.

[4] Jie Chu, Dawei Zhang. Adjustment on the disorder of intestinal flora of S. boulardii [J]. China Animal Husbandry & Veterinary Medicine, 2008, 35 (7): 87-89.

[5] Lianxiang Du, Fuping Lu, Microbiology experimental techniques[M]. Beijing: Light Industry Press in China, (2008).

[6] Deqing Zhou. Microbiology laboratory manual [M]. Shanghai: Shanghai Science and Technology Press, (1986).

[7] Kalmokoff ML, Ingledew WM (1985) Evaluation of ethanol tolerance in selected Saccharomyces strains. ASBC J 43: 189-196.

[8] Howieson J G, Ewing M A. Acid Tolerance, in the Rhizobium meliloti-Medicago Symbiosis [J]. Australian Journal of Agricutural Research, 1986, 37(1): 55.


[9] Kirchhelle A, Fruhwein N, Toburen D. Treatment of persisten diarrhea with S. boulardii in returning travelers. Results of prospective study [J]. Fortschr Med, 1996, 114(11): 136.