Study on Lipoprotein Lipase Production by Candida rugosa

Abstract:

Article Preview

Candida rugosa can produce lipoprotein lipase by fermentation in the medium on a rotary shaker, the optimal medium compositions were determined as, the optimum pH was 6.0, the contant was 25ml, the inoculum age of strain was 24h, the inoculation was 10%,the rotation speed was 200r/min and the strain was cultured 27h at 28°C.Under the optimum cultivation conditions, the maximum enzyme activity reached 121.65U/L.The result of enzyme properties showed, the optimum temperature and pH were 45°C and 7.5 separately.

Info:

Periodical:

Advanced Materials Research (Volumes 343-344)

Edited by:

David Wang

Pages:

604-610

DOI:

10.4028/www.scientific.net/AMR.343-344.604

Citation:

X. X. Zhao and Y. G. He, "Study on Lipoprotein Lipase Production by Candida rugosa", Advanced Materials Research, Vols. 343-344, pp. 604-610, 2012

Online since:

September 2011

Export:

Price:

$35.00

[1] Wang wei, Xu weihua, Han chunsheng, Fu si, Study on the Correlation of hyperlipidemia, hypertension and diabetes, Chinese modern medicine magazine, vol. 2, 2008, pp.47-48.

[2] Zhou yunfeng, Guan youfei, Study on the mechanism of Nephrotic syndrome and hyperlipidemia, progress in physiological sciences, vol. 39, 2008, pp: 67-90.

[3] Yao ying, Liu xiaocheng, Clinical observation of Xuezhikang on patients of nephrotic syndrome with hyperlipiderni.

[4] Raman P., Yadav, Rajrndra K, Saxena. Purification and characterization of a regiospecific lipase from aspergillus terreus, Biochem, vol. 28, 1998, pp.243-249.

[5] Roberto Arreguin-Espinosa, Purification and properties of a lipase from Cephaloleia presignis(Coleoptera, Chrysomelidae) , Biochem, vol. 31, 2000, pp.239-244.

DOI: 10.1042/ba19990088

[6] Rollof,J.,S.A. Hedstrom and P. Nilsson-Ehle, Lipolytic activity of Staphylococcus aureus strains from disseminated and localized infections, Microbiol. Immunol, vol . 95, 1987, pp.109-113.

DOI: 10.1111/j.1699-0463.1987.tb03096.x

[7] Sharma, R., Y. Chisti and U.C. Banerjee, Production, purification, characterization and applications of lipases, Biotechnol. Adv., vol . 19, 2001, pp.627-662.

DOI: 10.1016/s0734-9750(01)00086-6

[8] St Leger, R.J., J.O. Nelson and S.E. Screen, The entomopathogenic fungus Metarhizium anisopliae alters ambient pH, allowing extracellular protease production and activity, Microbiology, vol . 145, 1999, pp.2691-2699.

DOI: 10.1099/00221287-145-10-2691

In order to see related information, you need to Login.