Study on Lipoprotein Lipase Production by Candida rugosa
Candida rugosa can produce lipoprotein lipase by fermentation in the medium on a rotary shaker, the optimal medium compositions were determined as, the optimum pH was 6.0, the contant was 25ml, the inoculum age of strain was 24h, the inoculation was 10%,the rotation speed was 200r/min and the strain was cultured 27h at 28°C.Under the optimum cultivation conditions, the maximum enzyme activity reached 121.65U/L.The result of enzyme properties showed, the optimum temperature and pH were 45°C and 7.5 separately.
X. X. Zhao and Y. G. He, "Study on Lipoprotein Lipase Production by Candida rugosa", Advanced Materials Research, Vols. 343-344, pp. 604-610, 2012