In order to research effects of storage conditions on physical properties of cut tobacco, storage temperature, storage humidity and storage time were studied by uniform design method in this paper. Intuitive analysis results showed that the best store process parameters such as storage temperature, humidity and time were 26°C, 61% and 32h respectively. Stepwise regression analysis results indicated storage process parameters affected physical properties of cut tobacco such as moisture content, filling power, proportion of long strands and its conversion extremely. And the regression equations were significant effective. But there was no efficacious regression equation between proportion of small strands of cut tobacco and storage process parameters.