EM Biodegrading Characteristics of Petroleum Hydrocarbon in Artificial Seawater


Article Preview

In this study, the feasible condition for diesel oil degradation of EM was investigated in artificial seawater medium. Under the optimal growth and degradation condition of pH 8.0, 30°C, inoculation amount of 3%, substrate concentration of 0.5%, and NaCl concentration of 20 g/L, the n-alkanes of diesel oil was degraded in high efficiency with total degrading rate of 72.3%, and the average n-alkanes (C10- C21) removal could reach 74.8% within 15 days. The results indicated that EM could be used in the bioremediation of marine oil pollution.



Advanced Materials Research (Volumes 356-360)

Edited by:

Hexing Li, Qunjie Xu and Daquan Zhang




Z. Zhang et al., "EM Biodegrading Characteristics of Petroleum Hydrocarbon in Artificial Seawater", Advanced Materials Research, Vols. 356-360, pp. 1145-1151, 2012

Online since:

October 2011




[1] R. Tecon, S. Beggah, K. Czechowska, V. Sentchilo , P. M. Chronopoulou, T. J. Mcgenity and J. R. Van Der Meer. Development of a multistrain bacterial bioreporter platform for the monitoring of hydrocarbon contaminants in marine environments. Environ. Sci. Technol. 2010, 44, 1049–1055.

DOI: https://doi.org/10.1021/es902849w

[2] S. Cappello , G. Caruso, D. Zampino, L.S. Monticelli, G. Maimone1, R. Denaro, B. Tripodo, M. Troussellier, M. Yakimov and L. Giuliano. Microbial community dynamics during assays of harbour oil spill bioremediation: a microscale simulation study. Journal of Applied Microbiology. 2007, 102 , 184–194.

DOI: https://doi.org/10.1111/j.1365-2672.2006.03071.x

[3] Y.J. Huang, Z.B. Jiang, J. N. Zeng, Q. Z. Chen, Y.Q. Zhao, Y.B. Liao, L. Shou, X.Q. Xu. The chronic effects of oil pollution on marine phytoplankton in a subtropical bay, China. Environ Monit Assess. 2011, 176: 517–530.

DOI: https://doi.org/10.1007/s10661-010-1601-6

[4] J. González, F. G. Figueiras, M. Aranguren-Gassis, B.G. Crespo, E. Fernández, X. A. G. Morán, and M. Nieto-Cid. Effect of a simulated oil spill on natural assemblages of marine phytoplankton enclosed in microcosms. Estuarine, Coastal and Shelf Science, 2009, 83(3), 265–276.

DOI: https://doi.org/10.1016/j.ecss.2009.04.001

[5] D. R. Kuroda. Progress using bioremediation for site restoration by the US Army Corps of Engineers. Stud. Environ. Sci. 1997, 66, 393-404.

[6] R.C. Prince, T.F. Parkerton, and C. Lee. The primary aerobic biodegradation of gasoline hydrocarbons. Environ. Sci. Technol. 2007, 41, 3316-3321.

DOI: https://doi.org/10.1021/es062884d

[7] T.K. Dutta , and S. Harayama. Fate of crude oil by the combination of photooxidation and biodegradation. Environ. Sci. Technol. 2000, 34, 1500-1505.

DOI: https://doi.org/10.1021/es991063o

[8] K. Sugiura, M. Ishihara, T. Shimauchi, and S. Harayama. Physicochemical properties and biodegradability of crude oil. Environ. Sci. Technol. 1997, 31, 45-51.

DOI: https://doi.org/10.1021/es950961r

[9] A. Khaliq, M. Kaleem Abbasi, and T. Hussain. Effects of integrated use of organic and inorganic nutrient sources with effective microorganisms (EM) on seed cotton yield in Pakistan. Bioresource Technology. 2006, 97, 967-972.

DOI: https://doi.org/10.1016/j.biortech.2005.05.002

[10] Y. Xue, S.Q. Li. Immobilized EM bacteria refining wastewater processing. Applied Chemical Industry , In Chinese. 2010, 39(5), 727-731.

[11] X.J. Xiong, X.T. Cao. Application of effective microorganism in the biological wastewater treatment process. Environmental Sanitation Engineering , In Chinese. 2007, 15(3), 11-14.

[12] W.L. Zhu, Chao Wang. Experimental study of EM in wastewater biodegradation. Environmental Engineering, In Chinese. 2001, 19(6), 15-17.

[13] X.X. Zhang, W.L. Wu, Y.B. Zhang, J.C. Wang, Q.Y. Liu, C.X. Geng, and J. Lu. Screening of efficient hydrocarbon-degrading strains and study on influence factors of degradation of refinery oily sludge. Ind. Eng. Chem. Res. 2007, 46, 8910-8917.

DOI: https://doi.org/10.1021/ie061376+

[14] S.Y. Tian, J. Wang, L.H. Nie. Effect of two kinds of nitrogen sources on biodegradation of diesel oil by hydrocarbon degrading bacteria consortium. Marine Environmental Science, In Chinese. 2009, 28(1), 26-29.

[15] L.Q. Yang, J.H. Jiang. The microorganism degradation mechanism of petroleum hydrocarbons. Environmental Protection of Oil & Gas Fields, In Chinese. 2011, 21(2), 24-26.

[16] M.T. Bao, Q.G. Chen, X.N. Fan, P.Y. Sun, and Z.H. Gao. Isolation of hydrocarbon degradation bacteria and optimization of degradation condition. Periodical of Ocean University of China , In Chinese. 2010, 40(6), 115-120.

[17] Y. Su, C. Li, Z.P. Liu. Research on characteristics of a marine oil-degrading strain. Research of Environmental Sciences , In Chinese. 2008, 21(5), 32-36.

[18] X.X. Zhang, M.S. Zheng, R.J. Wang, C.F. Liang, and S. Xu. Screening of efficient hydrocarbon degrading bacteria Y-16 from petroleum contaminated soil and its degradation characteristics. Chinese Journal of Environmental Engineering , In Chinese. 2010, 4(8): 1716-1720.

[19] C.Y. Wang, Y.S. Ding. Isolation of crude oil degrading strains and their degradation capability. Journal of Dalian Maritime University, In Chinese. 2008, 34(3): 9-12.

[20] M.M. Liu, L.H. Jin, W.S. Li, and Y.Y. Jia. Study on immobilization of an oil-degrading strain a on activated carbon fiber. Environmental Pollution and Cnntrol, In Chinese. 2009, 31(10): 49-51.

[21] K.P. Xie, J.T. Zhou, Y.Y. Qu, M.J. Xie, H.T. Wang ,Y.X. Li, and Y. Ke. Studies on isolation,identification of halophil crude oil degrading bacteria and their degradation capability. Marine Environmental Science, In Chinese. 2009, 28(6): 680-683.

[22] X.R. Ye, J.X. Shi, Z.Y. Chen. Gas chromatography analysis for the marine bacteria degrading effect of petroleum hydrocarbon. Journal of Instrumental Analysis, In Chinese. 2007, 26(6): 847-850.

[23] H.F. Santos, F.L. Carmo, J.E.S. Pages, A.S. Rosado, and R.S. Peixoto. Bioremediation of mangroves impacted by petroleum. Water Air Soil Pollut , 2011, 216: 329–350.

DOI: https://doi.org/10.1007/s11270-010-0536-4

[24] Y. Huang, X. Li, J.L. Cai. Review on bioremediation of oil contamination. Ecology and Environmental Sciences, In Chinese. 2009, 18(1): 361-367.

[25] M.A. Zahed, H.A. Aziz, M.H. Isa, and L. Mohajeri. Enhancement Biodegradation of n-alkanes from Crude Oil Contaminated Seawater. Int. J. Environ. Res. 2010, 4(4): 655-664.