This paper presents a systematic study of the biometric leaching sequencing batch experiment. The major process parameters include the inoculation amount of bacteria(5%) and substrate concentration(4g/L)(elemental sulfur). The chemical speciation transformation takes place in Biology leaching were measured by sequential extraction techniques. Through which before and after the leaching we can Analyze the filter mechanism and leaching ability. According to Cd, Pb content of each chemical speciation before and after the leaching, the release rules of different chemical speciation for each elements in every sampling period were calculated. This paper also analyzed the leaching ability of different chemical speciation in different leaching condition. The results indicated that,Cd drains out through the indirect mechanism, Pb drainings is mainly completes by direct machine-made and the indirect mechanism combined action.