Micro Tom is a novel breed model organism of Lycopersicon esculentum Mill. It has the a short life cycle and is dwarf and miniature. Owing to these characters, the utilization of Micro Tom can decrease the period of scientific research greatly and save time and space for breeding, so its application is more and more broad. In this paper, we successfully induced the regenerated seedlings from Micro Tom to bear fruits in advance by controlling and optimizing several external conditions such as the initial height of the regenerated seedlings, the culture medium, the photoperiod and the temperatures, etc. Before maturity induction, we firstly investigated the proper methods for plantlets regeneration and got a relative complete data. In the later investigation on precocity inducing we found that the optimum inducing culture medium is MS medium supplemented with 0.1 mg/L NAA and 0.5 mg/L IBA, the proper height of seedlings is 2.0-2.5 cm, the best photoperiod is 18-20h, and the most efficient culturing temperature is 22-26°C. In this way we can get regenerated seedlings from Micro Tom in an efficient way and make them precocity in vitro.