Preparation and Characterize of Konjac Glucomannan-Based Protein Molecularly Imprinted Polymer

Abstract:

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Objective: To prepare a novel molecularly imprinted materials for protein recognition. Methods: Konjac glucomannan was used as fundamental materials, after swollen, added 3-Chloro-1,2- epoxypropane and glycerol cross-linked for 3 h at 40 °C. Then added bovine serum albumin (BSA) solution, agitated 1 h at room temperature. Natural dried. Results: After eluted, the imprinted efficiency of the imprinted membrane were (59.27±3.53) % (n=6) with blank membrane as control. The adsorption rate increased from 2 h to 20 h, and reached saturation at 22 to 24 h. The selectively recognition experiment indicated the BSA imprinted membrane have significant selectively recognition abilities for the template protein. Conclusions: konjac glucomannan-based imprinted membrane shows great possibilities to be a promising material for protein molecularly imprinted recognition.

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Periodical:

Edited by:

Helen Zhang and David Jin

Pages:

460-463

DOI:

10.4028/www.scientific.net/AMR.366.460

Citation:

Z. Y. Mi et al., "Preparation and Characterize of Konjac Glucomannan-Based Protein Molecularly Imprinted Polymer", Advanced Materials Research, Vol. 366, pp. 460-463, 2012

Online since:

October 2011

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Price:

$35.00

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