Abstract: Effect of Galaeto-oligosaccharide (GOS) and Isomalto-oligosaccharide (IMO) on growth of Lactobacillus acidophilus, Bifidobacterium longum and L. rhamnosus was studied. The addition of each substance (w/v) was 0.0%, 0.1%, 0.5%, 1.0% 1.5% and 2.0%.The methods were analyzed titratable acidity (°T), pH and enumeration that using reconstituted skim goat milk media as the control. All samples were incubated at 37 C for 18h. Results were as follows: addition of GOS has the significant inhibition on the growth of L. acidophilus and promotion on the growth of L. rhamnosus, while the effect on the growth of B. longum was not obviously. Addition of IMO could promote the growth of L. acidophilus, especially in low level. The growth of B. longum was increased with increase in IMO. While the effect on the growth of L. rhamnosus by adding IMO was inhibited and obviously in the high concentration.
Abstract: Four different prebiotics including fructo-oligosaccharide isomalto-oligosaccharide, inulin and xylo-oligosaccharide were screened for their effects on survival of Bifidobacterium bifidum during freeze-drying. The additions of prebiotics (v/v) were all 4%, 8%, 12%, 16% and 20%. The results showed that prebiotics could act on Bifidobacterium Bifidum. The optimum concentration of fructo-oligosaccharides (FOS) in samples for surival of Bifidobacterium bifidum was 12% and for viable count was 20％. Addition of xylo-oligosaccharides (XOS) played a significant role in promoting cell viablity when it was in low level. The optimum concentration of isomalto-oligosaccharides (IMO) for surival of Bifidobacterium bifidum was 16% and for viable count was 12％, while inulin in samples for surival and viable cell of Bifidobacterium bifidum both was 4％.
Abstract: According to the problem of mine water bursting frequent accidents when Mine into the deep mining. Based on the theory of fluid-solid coupling, accounting for the effect on permeability by damage and stress. Analysis of influence factors what hydraulic fracturing process, fracture propagation pattern, and influencing factors including shape and magnitude of inlet hole, stress conditions, and specimen strength were investigated. Hydraulic fracturing is not only a natural action but also an artificial method to alter rock structure. Application of rock failure process analysis software coupled seepage-stress F-RFPA2D, numerical simulated rock water pressure to cause crack rupture instability process, research the fracture law of the rock on water pressure and vertical loading. Combine similar physical experiment model, contrast analysis of two broken results and stress-strain curve, reveals instability mechanical behavior of rock hydraulic fracture process. Obtain deep mining in the process of mine water seepage and water extrude, overburden rock crack up, expand, water seepage, water extrude, instability rupture process rules. numerical simulation results and experimental results has good uniformity, for disaster of the mine water extrude has immeasurable prediction practical.
Abstract: At present, ceramic coatings have attracted more and more attention for its good resistance, good chemical stability, and high temperature resistant, hydrophobic and other properties. SiO2 sol was prepared by chelated silicon powders to prepare SiO2 sol-gel film on the nickel plating layer. Pre-plating ordinary bright nickel-plating layer and composite bright nickel plating layer on the iron surface in advance, research some properties such as abrasion resistance, porosity, and NaCl solution soak test of SiO2 sol-gel films by changing the times of immersing SiO2 sol and baking temperature after immersing. The results show that: The time of emerging rust points for sol-gel coating on composite bright nickel-plated is much longer than that on ordinary bright nickel-plated in salt water soak test. The porosity of sol-gel coatings on composite bright nickel-plated is lower than that on ordinary bright nickel-plated. When the drying temperature is at 400°C or 500°C, the porosity rate is zero for the composite bright nickel without coatings or coated with any times of sol. It can improve wear resistance of the composite bright coating when immersing SiO2 sol at 500°C for 3 to 5 times. Porosity of SiO2 sol-gel coatings on the ordinary bright nickel layer is zero when baking at 500°C, immersing 3 times or more than 3 times. The best drying temperature is at 500°C, the best immersing times is 4 times to obtain better corrosion resistance for the bright nickel deposits. The color of the better appearance for a film is rainbow, its drying temperature of 500°C, immersing 4 times.
Abstract: Bridge structure has important impact on pavement stress, and previous research often analyses separately which is one of the reason for pavement disease. This paper analyses the sensitivity of structure in girder steel bridge deck pavement with the finite element method, obtaining the changing regularity of maximum transverse strain, maximum longitudinal strain, maximum longitudinal displacement of pavement surface, interlaminar shearing stresses with six kinds of the structure design parameters including bridge roof slab thickness, diaphragm plates distance, U rib width, distance, thickness, girder space under the most unfavorable load. The study can offer help for the determination of bridge structure design parameters.
Abstract: Commonly the vibration due to vehicle loads has no apparently impact on highway bridges, but it is unneglectable when the heavy vehicles load on highway bridges. The impact factor is usually used to define the dynamic effect under vehicle loads in most design code. In this paper, the models of simple composite box beams with different span and the models of two simplified heavy vehicles are established respectively. The impact factors are calculated when the heavy loads pass though bridges at different speed under different load conditions. In addition, the change laws of the impact factors and the influence of different vehicle models on the impact factors are analyzed. Analysis results show that, not only the impact factor are increased with vehicle speed, but also the amplitude and period are all increased. In normal speed range, the influence of speed on the impact factors appears rising trend overall. For the bridge with same span, the impact factors under the double wheel load are smaller than that under single wheel load.
Abstract: One geminal dicationic ionic liquid (IL) was synthesized and coated in column as stationary phase for capillary gas chromatography (CGC). In this study, two times imidazolium cations were reacted with 1,4-bis(chloromethyl)-benzene and then the anions exchanged to bis(trifluoromethylsulfonyl)-imide (NTf2-). The new ILs was coated statically in capillary column. The outcome of the stationary phase was evaluated by separating Grobs mixture, n-alkanes mixture, alcohols mixture and aromatic position isomers mixture such as xylene, nitrotoluene, dichlorobenzene and cresol. The results indicated that new IL possessed good column coating properties and chromatographic separation abilities.
Abstract: This study investigated the effects of exogenous estradiol on sex reversal in the Asian swamp eel (Monopterus albus) by evaluating the estradiol (E2) and testosterone (T) concentrations, gonadosomatic index (GSI), hepaticosomatic index (HSI) and the sex ratio. Fish (9.60 ± 0.92 g average initial weight; n = 3 per group) were fed with six different diets containing 0 (control), 20, 40, 60, 80 or 100 mg kg-1 dietary estradiol contents to apparent satiation for 15 months. Serum E2 and T concentrations were determined by radioimmunoassay. Levels of E2, GSI and HSI significantly increased while T concentrations and survival rates decreased as the exogenous estradiol levels were raised. Sex ratios did not differ significantly among all exogenous estradiol treatments, and there were no significant differences between the control group and the 20 mg kg-1 treatment group. Therefore, exogenous estradiol can maintain the female activity and delay sex reversal in M. albus. This study provides important information for successful reproductive management and may be exploited for the aquaculture of this species.
Abstract: This study investigated the effects of busulfan on sex reversal in the Asian swamp eel (Monopterus albus) by evaluating the estradiol (E2) and testosterone (T) concentrations, gonadosomatic index (GSI) and sex ratio. Fish (9.80 ± 1.07 g average initial weight; n = 3 per group) were fed with five diets containing 0 (control), 10, 30, 50 or 70 mg kg-1 dietary busulfan concentrations to apparent satiation for 15 months. Serum E2 and T concentrations were determined by radioimmunoassay techniques. Both E2 and T concentrations were significantly affected by busulfan, while there were no significant differences among the treated groups. Serum E2 concentrations and GSIs decreased, while T concentrations increased with increasing busulfan levels in each group. A shift in the sex ratio towards more male and intersex fish was observed with increasing busulfan contents. The highest male ratio obtained in the 70 mg kg-1 group was significantly different compared to the other groups. High doses of busulfan may induce deformed individuals and high mortality in M. albus. Therefore, busulfan may inhibit gonad development and promote the sex change from female to male M. albus.