Preparation and Immunological Traits of Monoclonal Antibody against Sarafloxacin

Abstract:

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Through cell fusion technology, five hybridoma lines of sarafloxacin (SAR), named S1-B2, S2-C6, S2-E7, S3-C5, and S3-E5, were identified and their corresponding mAbs were of the IgG1 isotype with a k light chain. The Kaffs of all mAbs were between 2.8 and 4.6×109 L/mol. The titers and IC50 values of purified ascite fluids were in the range of 0.512–2.56×106 and 0.32–0.48 ng/mL, respectively. The performances of S1-B2 and S2-C6 were more consistent in the stability experiments. Based on the S1-B2 hybridoma, an icELISA method was developed. The dynamic range was from 0.004 to 18 ng/mL, with a detection limit for the assay and IC50 values of 0.002 and 0.32 ng/mL, respectively. Therefore, the establishment of these hybridomas may provide an alternative method for the detection of SAR residues in food-original animals.

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Periodical:

Edited by:

Helen Zhang and David Jin

Pages:

54-57

DOI:

10.4028/www.scientific.net/AMR.459.54

Citation:

G. Y. Fan and J. Q. Jiang, "Preparation and Immunological Traits of Monoclonal Antibody against Sarafloxacin", Advanced Materials Research, Vol. 459, pp. 54-57, 2012

Online since:

January 2012

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Price:

$35.00

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