In this work, the effect of multiwalled carbon nanotubes (NWNTs) incorporation in poly(L-lactide) (PLLA) on the human osteoblast-like cell (Saos-2) growth behaviour was studied. The PLLA/MWCNT composite with different ratio of PLLA to MWCNT was fabricated using solvent-casting technique. MWCNT was detected by XRD and showed the successful incorporation of MWCNT into PLLA. Fixed amount of cells were cultured on different composite. Cell viability assay and scanning electron microscopy were used to evaluate cell adhesion, morphology and cytoskeleton development on the scaffolds. The activity of mitochondrial dehydrogenase (MD) enzyme was evaluated by MTT substrate and p-nitrophenyl phosphate substrate. The results demonstrated cytoskeleton fibre and good adhesion of osteoblast cells to the composite surface. No significant differences of cell viability were found for the PLLA/MWCNT composite and controls. The incorporation of MWCNT into PLLA can obviously enhance the MD activity. We believe that sample with more MWCNT provided favourite interactions to the human osteoblast-like cells, which indicate a potential candidate in bone tissue engineering.