Fabrication of Poly(L-Latic Acid) Scaffolds with Wool Keratin for Osteoblast Cultivation
As a natural protein, wool keratin was used to improve the cell affinity of poly(L-lactic acid) (PLLA). Small keratin particles were prepared from keratin solution by the spray-drying process. Keratin particles were blended with PLLA/1,4 dioxane solution and paraffin micro-spheres which were used as progens. After the mixture was molded and dried, the paraffin micro-spheres were removed by cyclohexane. PLLA/keratin scaffolds with controlled pore size and well interconnectivity were fabricated. Keratin releasing rate was detected by Fourier transform infrared (FTIR) after the scaffold was immersed into PBS up to 4 weeks. The surface chemical structure was examined by X-ray photoelectron spectroscope (XPS). The results suggested that the keratin could be held into the scaffold which was expected to improve the interactions between osteoblasts and the polymeric scaffolds.
Alan K.T. Lau, J. Lu, Vijay K. Varadan, F.K. Chang, J.P. Tu and P.M. Lam
J. S. Li et al., "Fabrication of Poly(L-Latic Acid) Scaffolds with Wool Keratin for Osteoblast Cultivation", Advanced Materials Research, Vols. 47-50, pp. 845-848, 2008