Smart Hybrid Mussel Adhesive Materials for Cell and Tissue Engineering
Mussel foot protein is widely known for candidate of strong adhesive materials. Six times fp-1 decapeptide repeats and fp-5 are chosen among the various type of foot protein and fused into (MAP) fp-151. Through various cell-adhesion analyses, we previously demonstrated that fp- 151 has the potential to be used as a cell or tissue bioadhesive. In the present study, to improve the cell-adhesion properties of fp-151, it was designed a new cell-adhesive protein, fp-151-RGD, which is a fusion with the GRGDSP residues, a RGD peptide sequence that has previously been identified at the cell-attachment site of fibronectin, at the C-terminus of fp-151. Also, recombinant fp-151- RGD maintained the advantages associated with fp-151, such as a high production yield in Escherichia coli and simple purification, it showed superior spreading ability, which is important for cell proliferation under serum-free conditions, as well as better cell-adhesion ability compared with other commercially produced cell-adhesion materials such as poly-L-lysine (PLL) and the naturally extracted MAP mixture Cell-Tak. The excellent adhesion and spreading abilities of fp- 151-RGD might be due to the fact that it utilizes three types of cell-binding mechanisms: DOPA adhesion of Cell-Tak, cationic binding force of PLL, and RGD sequence-mediated adhesion of fibronectin. Therefore, the new recombinant fp-151-RGD is suitable for use as a cell-adhesion material in cell culture or tissue engineering, and in any other area where efficient cell adhesion is required.
Alan K.T. Lau, J. Lu, Vijay K. Varadan, F.K. Chang, J.P. Tu and P.M. Lam
D. S. Hwang et al., "Smart Hybrid Mussel Adhesive Materials for Cell and Tissue Engineering", Advanced Materials Research, Vols. 47-50, pp. 861-864, 2008