Characterization of Eugenol Extracted from Lemongrass (Cymbopogon citratus (DC.) Stapf) for Food Packaging Materials
The goal of this research was to study a prospect of applying Lemongrass (Cymbopogon citratus (DC.) Stapf) extracted having antioxidant property (i.e. eugenol) to replace commercial antioxidant agent such as butylated hydroxytoluene (BHT) in packaging raw materials. The extracted eugenol was characterized using Gas chromatography (GC). A free radical scavenging activities of eugenol and BHT were also investigated using 2, 2-dipheny l-1-picryhydrazyl (DPPH) assay. It reveals that a concentration of eugenol resulting in a 50% inhibition of the free radical, IC50, (0.11 mg/ml) is lower than the IC50 value of BHT (0.14 mg/ml) indicating better radical scavenging activity. In its radical form, DPPH shows an absorbance maximum at 515 nm which disappears upon reduction by an antiradical compound. BHT, a synthetic antioxidant, slowly reacts with DPPH reaching a steady state within 5 hr. The kinetic (R2) is estimated to be 0.9283 at 25 °C. Eugenol rapid reacts with DPPH reaching a steady state within 2 h. The kinetic (R2) is estimated to be 0.9946 at 25 °C, our results conrm that eugenol can we used instead of BHT.
N. Petchsoongsakul and C. Pechyen, "Characterization of Eugenol Extracted from Lemongrass (Cymbopogon citratus (DC.) Stapf) for Food Packaging Materials", Advanced Materials Research, Vol. 506, pp. 603-606, 2012