Effect of Alginate and Cell Suspension on Viable Counts and Efficacy of Entrapment of Encapsulated B. bifidum BB28
This study reports the encapsulation yield (EY) of B. bifidum BB28 encapsulated in different sodium alginate concentrations and in different proportion of BB28 and sodium alginate.It was studied by enumeration of viable cells after depolymerization of the capsules in 9mL trisodium citrate, followed by using MRS agar at 37°C for 48 h. The concentrations of sodium alginate was 1.5%, 2.0%, 2.5% and 3.0%.The proportion of cell suspension and sodium alginate was 1:5,1:10,1:15 and 1:20. Results were as follows: The optimum concentration of sodium alginate concentrations was 2.5% and the optimum proportion of cell suspension and sodium alginate was 1:10.
Helen Zhang and David Jin
H. Chen et al., "Effect of Alginate and Cell Suspension on Viable Counts and Efficacy of Entrapment of Encapsulated B. bifidum BB28", Advanced Materials Research, Vol. 531, pp. 499-502, 2012