Extraction and Purification of Polyphenolic Compounds Obtained from Hsian-Tsao (Mesona Procumbens Hemsl.)


Article Preview

Extraction and purification of polyphenolic compounds from Hsian-tsao (Mesona procumbens Hemsl.) were investigated. The polyphenolic compounds were extracted by using acetone, ethanol or methanol at three different concentrations. The polyphenolic concentration and the inhibition of lipid peroxidation were determined. The results showed that, among solvents tested, 75% acetone solvent extracted a maximum amount of polyphenolic compounds from Hsian-tsao. The 75% acetone extract was purified further by AB-8 adsorption column chromatography and eight fractions, A~H, were obtained. Among eight fractions, Fraction E had the highest polyphenolic content and inhibition(76%). Moreover, Fraction E was separated into four polyphenolic compounds (1~4) by HPLC and each compound reached a purity of more than 98%. The structure of the compound was identified by LC-MS and NMR, and the compound-4 was determined to be astragalin (a flavonol glycoside) which was obtained from HT for the first time.



Advanced Materials Research (Volumes 550-553)

Edited by:

Zili Liu, Feng Peng and Xiaoguo Liu




Z. X. Huang et al., "Extraction and Purification of Polyphenolic Compounds Obtained from Hsian-Tsao (Mesona Procumbens Hemsl.)", Advanced Materials Research, Vols. 550-553, pp. 1790-1795, 2012

Online since:

July 2012




[1] F. Oke, B. Aslim, S. Ozturk and S. Altundag, Essential oil composition, antimicrobial and antioxidant activities of Satureja cuneifolia Ten., Food Chem., 112 (2009) 874-879.

DOI: https://doi.org/10.1016/j.foodchem.2008.06.061

[2] L.M. Yu, M.M. Zhao, J.S. Wang, Antioxidant, immunomodulatory and anti-breast cancer activities of phenolic extract from pine (Pinus massoniana Lamb) bark, Innov. Food Sci. Eerg. Tech., 9 (2008) 122-128.

DOI: https://doi.org/10.1016/j.ifset.2007.06.006

[3] C.Y. Hung and G.C. Yen, Antioxidant activity of phenolic compounds isolated from Mesona procumbens Hemsl. J. Agr. Food Chem., 50 (2002) 2993-2997.

[4] G.C. Yen, P.D. Duh and Y.L. Hung, Contributions of major components to the antimutagenic effect of Hsian-taso (Mesona procumbens Hemsl. ), J. Agr. Food Chem., 49 (2001) 5000-5004.

DOI: https://doi.org/10.1021/jf0103929

[5] U.D. Chavan, F. Shahidi and M. Naczk, Extraction of condensed tannins from beach pea (Lathyrus maritimus L. ) as affected by different solvents Food Chem., 75 (2001) 509-512.

DOI: https://doi.org/10.1016/s0308-8146(01)00234-5

[6] N. Trukmen, F. Sari and Y. S. Velioglu, Effects of extraction solvents on concentration and antioxidant activity of black and black mate tea polyphenolics determined by ferrous tartrate and Folin-Ciocalteu methods, Food Chem., 99 (2006) 835-841.

DOI: https://doi.org/10.1016/j.foodchem.2005.08.034

[7] J.L. Fan, W.X. Zhu, H.B. Kang, Flavonoid constituents and antioxidant capacity in flowers of different Zhongyuan tree penoy cultivars, J. Funct. Foods, 4 (2012) 147-157.

DOI: https://doi.org/10.1016/j.jff.2011.09.006

[8] C.Y. Hung and G.C. Yen, Extraction and identification of antioxidative components of Hsian-tsao (Mesona procumbens Hemsl. ), Lebensm. -Wiss. u. -Technol., 34 (2001) 306-311.

DOI: https://doi.org/10.1006/fstl.2001.0775

[9] S.Z. Zhang, X.N. Yang, R.A. Coburn and M. E. Morris, Structure activity relationships and quantitative structure activity relationships for the flavonoid-mediated inhibition of breast cancer resistance protein, Biochem. Pharmacol., 70 (2005).

DOI: https://doi.org/10.1016/j.bcp.2005.05.017

[10] A. Palida, R. S. Mi, Y. Y. Cong, et al, Isolation and identification of chemical constituents in Ocimum bacilicum L., West China J. Pharm. Sci., 22 (2007) 489-490.