Application of the Enzymolysis and Membrane Technology in Glucan Extraction and Purification from Grifola frondosa


Article Preview

It has been investigated that the application of the enzymolysis and membrane technology in the extraction and purification of glucan from Grifola frondosa. The fruitbody of Grifola frondosa was been used to extract polysaccharides. Compared the yield, protein content and polysaccharide content in the extracts under 3 different process modes, it was found that the procedure processed by complex enzyme was better than the others. Microfiltrated with membrane of 0.2μm aperture and then ultrafiltrated with membrane with molecular weight cutoff of 1000 Dalton, it had glucan retained rate of 80.42% and protein removed rate of 88.27%. GCP experiment indicated that the glucan get from the procedure was a homogeneous polysaccharide with weight average molecular weight of 130355 Dalton. The index of molecular weight distribution was 1.1844. The desalination rate was increased by adding water to the concentrate while ultrafiltration was in progress. When the quantity of water added was 1.5 times of the concentrate volume, it had effective desalination effect with the ash content of 3.79% in final product.



Advanced Materials Research (Volumes 581-582)

Edited by:

Jimmy (C.M.) Kao, Wen-Pei Sung and Ran Chen




C. B. Zhou et al., "Application of the Enzymolysis and Membrane Technology in Glucan Extraction and Purification from Grifola frondosa", Advanced Materials Research, Vols. 581-582, pp. 11-14, 2012

Online since:

October 2012




[1] M Zhang, SW Cui, CK Cheung, et al. Trends in Food Science & Technology Vol. 18 (2007), pp.4-19.

[2] BCh Lee, JT Bae, HB Pyo, et al. Enzyme and Microbial Technology Vol. 32 (2003), pp.574-581.

[3] Suzuki I, Hashimoto K, Oikawa S, et al. Chemical & Pharmaceutical Bulletin Vol. 37 (1989), pp.410-413.

[4] Misaki A, Kututa M, Sasaki T, et al. Carbohydrate Research Vol. 92(1981), pp.115-129.

[5] GH Zhao, ZD Chen, ZhX Li. Food and Fermentation Industries Vol. 21 (2001), pp.45-48.

[6] TS Ma, G Zuazaga. Industrial & Engineering Chemistry: Analytical Addition Vol. 14 (1942), pp.280-282.

[7] H Qian, T Zhang, ChH Liu. Jiangsu Food and Fermentation Vol. 3 (2002), pp.28-30.

[8] ZP Xu, BQ Wang, YP Su. Physical Testing and Chemical Analysis Part B: chemical Analysis Vol. 47 (2011), pp.924-926, 929.