Efficient Expression of Mussel Adhesive Protein in Pichia pastoris GS115 Promoted by Ectoine
Mussel adhesive protein has significant potential application in the field of medical adhesion. Genetic engineering method is gaining more and more attention, by which mussel adhesive protein can be heterologously expressed. In order to improve the expression efficiency of mussel adhesive protein with heterologous recombinant, it is reported that the compatible solutes Ectoine promoted to expression of adhesive protein on Pichia pastoris GS115. In this study, the adhesive protein gene msfp-1 from Mytilus sp. JHX-2002 was transformed into P. pastoris GS115. Inducement expression of adhesive protein Msfp-1 with various methanol concentrations was investigated. The promotion of Ectoine on the expression level of recombinant protein was studied. The results showed that adhesive protein Msfp-1 was induced with methanol on the recombinant GS115/msfp-1. The optimal concentration of methanol was 1% on heterologous expression. In the inducement expression phase of Msfp-1 with methanol, Ectoine could play a promotion role on expression of heterologous proteins. When the concentration of methanol was 1.5% and the addition of Ectoine was 1.5 mM, the expression of Msfp-1 was up to 2.1 g/L. Compared to fermentation broth without Ectoine, the expression was increased by 61.5%. Ectoine has an important promotion in the efficient expression of mussel adhesive protein on P. pastoris GS115.
Jimmy (C.M.) Kao, Wen-Pei Sung and Ran Chen
X. Zheng et al., "Efficient Expression of Mussel Adhesive Protein in Pichia pastoris GS115 Promoted by Ectoine", Advanced Materials Research, Vols. 581-582, pp. 1137-1140, 2012