Preparation and Properties of Cross-Linked Enzyme Aggregates of Cellulase
A new carrier-free cross-linked aggregates of cellulase (CLEAs-C) via precipitation with ammonia sulfate and cross-linking with glutaraldehyde was prepared. Efficient enzyme activity was obtained when cellulase and glutaraldehyde concentration was 50mg/mL and 3% (v/v) respectively. The cross-linking time and temperature were also important parameters for immobilization. Optimal temperature and pH of the CLEA–C were evaluated. The CLEAs-C displayed good stabilities, after stored at 4○C for 28 days storage, the CLEAs retained more than 80% initial activities.
Jimmy (C.M.) Kao, Wen-Pei Sung and Ran Chen
B. Li et al., "Preparation and Properties of Cross-Linked Enzyme Aggregates of Cellulase", Advanced Materials Research, Vols. 581-582, pp. 257-260, 2012