Preparation and Properties of Cross-Linked Enzyme Aggregates of Cellulase

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A new carrier-free cross-linked aggregates of cellulase (CLEAs-C) via precipitation with ammonia sulfate and cross-linking with glutaraldehyde was prepared. Efficient enzyme activity was obtained when cellulase and glutaraldehyde concentration was 50mg/mL and 3% (v/v) respectively. The cross-linking time and temperature were also important parameters for immobilization. Optimal temperature and pH of the CLEA–C were evaluated. The CLEAs-C displayed good stabilities, after stored at 4○C for 28 days storage, the CLEAs retained more than 80% initial activities.

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Periodical:

Advanced Materials Research (Volumes 581-582)

Edited by:

Jimmy (C.M.) Kao, Wen-Pei Sung and Ran Chen

Pages:

257-260

Citation:

B. Li et al., "Preparation and Properties of Cross-Linked Enzyme Aggregates of Cellulase", Advanced Materials Research, Vols. 581-582, pp. 257-260, 2012

Online since:

October 2012

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$38.00

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