Nanostructured Hydroxyapatite-Chitosan Composite Biomaterial for Bone Tissue Engineering


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In the recent years, significant developments have been achieved with chitosan and hydroxyapatite (HAp) scaffolds for bone tissue engineering. In the present study, chitosan/nanostructured hydroxyapatite (Chitosan/nHAp) has been prepared and subsequently characterized physicochemically for bone graft substitution. The nano sized HAp particles were uniformly distributed in the chitosan matrix which was confirmed by Fourier Transform Infrared Spectroscopy, Thermal Gravimetric Analysis, X-Ray Diffraction and Scanning Electron Microscopy analysis. The pore size of the chitosan/nHAp scaffold was found to be 18-372 µm which is suitable for cell attachment and nutrient supplement. Thus, we are suggesting that Chitosan/nHAp could be promising biomaterials for bone tissue engineering.



Edited by:

D. Rajan Babu




J. Venkatesan and S. K. Kim, "Nanostructured Hydroxyapatite-Chitosan Composite Biomaterial for Bone Tissue Engineering", Advanced Materials Research, Vol. 584, pp. 212-216, 2012

Online since:

October 2012




[1] W.W. Thein-Han and Misra, R.D.K., Biomimetic chitosan-nanohydroxyapatite composite scaffolds for bone tissue engineering. Acta Biomaterialia, 5 (2009) 1182-1197.


[2] J. Venkatesan and Kim, S. -K., Chitosan composites for bone tissue engineering—An overview. Marine Drugs, 8 (2010) 2252-2266.


[3] J. Je and Kim, S., Water-soluble chitosan derivatives as a BACE1 inhibitor. Bioorganic & medicinal chemistry, 13 (2005) 6551-6555.


[4] Y. Jeon, Shahidi, F., and Kim, S., Preparation of chitin and chitosan oligomers and their applications in physiological functional foods. Food Reviews International, 16 (2000) 159-176.


[5] J. Venkatesan, et al., Preparation and characterization of carbon nanotube-grafted-chitosan - Natural hydroxyapatite composite for bone tissue engineering. Carbohydrate Polymers, 83 (2011) 569-577.


[6] R. Pallela, et al., Biophysicochemical evaluation of chitosan-hydroxyapatite-marine sponge collagen composite for bone tissue engineering. Journal of Biomedical Materials Research Part A, 100A (2012) 486-495.


[7] R. Jayakumar, et al., Novel chitin and chitosan nanofibers in biomedical applications. Biotechnology Advances, 28 (2010) 142-150.

[8] L.C. Palmer, et al., Biomimetic systems for hydroxyapatite mineralization inspired by bone and enamel. Chemical Reviews, 108 (2008) 4754-4783.

[9] S.M. Best, et al., Bioceramics: Past, present and for the future. Journal of the European Ceramic Society, 28 (2008) 1319-1327.

[10] E. Landi, et al., Carbonated hydroxyapatite as bone substitute. Journal of the European Ceramic Society, 23 (2003) 2931-2937.


[11] T.E. Orr, et al., Compressive properties of cancellous bone defects in a rabbit model treated with particles of natural bone mineral and synthetic hydroxyapatite. Biomaterials, 22 (2001) 1953-(1959).


[12] X. -J. Tang and et al., Hard tissue compatibility of natural hydroxyapatite/chitosan composite. Biomedical Materials, 3 (2008) 044115.

[13] H. Yuan, et al., Experimental study of natural hydroxyapatite/chitosan composite on reconstructing bone defects. Journal of Nanjing Medical University, 22 (2008) 372-375.


[14] J. Venkatesan, et al., A comparative study of thermal calcination and an alkaline hydrolysis method in the isolation of hydroxyapatite from Thunnus obesus bone. Biomedical Materials, 6 (2011) 035003.