Biodegradable Amphiphilic Tri-Block Copolymeric Nanoparticles for Controlled MTB Drug Delivery


Article Preview

. A series of biodegradable amphiphilic tri-block copolymers (PLGA–PEG–PLGA) have been derived from the diblock copolymer poly (lactic–co–glycolic acid (PLGA)) and polyethylene glycol (PEG). The mycobacterium tuberculosis (MTB) drug pyrazinamide (PZA) loaded polymer nanoparticles (NPs) have been prepared by probe-sonication followed by w/o/w double emulsification technique. The copolymers have been characterized by FTIR and 1HNMR spectroscopic techniques, TG-DTA analysis, GPC analysis and powder XRD pattern. The MTB drug loaded polymeric NPs have been characterized by FESEM, powder XRD, HRTEM and XPS analysis. The drug loading efficiency, drug content and in vitro drug release studies have been carried out by spectrophotometry. The drug loading efficiency and drug content of triblock copolymeric NPs were higher than these of diblock copolymeric microparticles (MPs). The in vitro drug release studies indicate that the NPs exhibit initial burst release followed by controlled release of PZA for longer durations. The drug release kinetics mechanism has been evaluated by zero order, first order, Korsemeyer-Peppas (KP) and Higuchi models.



Edited by:

D. Rajan Babu




M. Gajendiran and S. Balasubramanian, "Biodegradable Amphiphilic Tri-Block Copolymeric Nanoparticles for Controlled MTB Drug Delivery", Advanced Materials Research, Vol. 584, pp. 460-464, 2012

Online since:

October 2012




[1] Q.U. Ain, S. Sharma, G.K. Khuller S.K. Garg, Alginate-based oral drug delivery system for tuberculosis: pharmacokinetics and therapeutic effects, J. Antimicrob. Chemother. 51 (2003) 931–938.


[2] H. Tai, W. Wang, T. Vermonden, F. Heath, W.E. Hennink, C. Alexander, K.M. Shakesheff, S.M. Howdle, Thermoresponsive and photocrosslinkable PEGMEMA-PPGMA-EGDMA copolymers from a one-step ATRP synthesis, Biomacromolecules 10 (2009) 822–828.


[3] S.Y. Lee, H. Hyun, J.Y. Youn, B.S. Kim, I.B. Song, M.S. Kim, B. Lee, G. Khang, H.B. Lee, Preparation of nano-emulsified paclitaxel using MPEG–PLGA diblock copolymers, Colloids Surf. A 313–314 (2008)126–130.


[4] F. Ito, H. Fujimori, H. Honnami, H. Kawakami, K. Kanamura, K. Makino,  Effect of polyethylene glycol on preparation of rifampicin-loaded PLGA microspheres with membrane emulsification technique, Colloids Surf. B 66 (2008) 65–70.


[5] P. Lan, Y. Zhang, Q. Gao, H. Shao, X. Hu, Studies on the synthesis and thermal properties of copoly(L-lactic acid/glycolic acid) by direct melt polycondensation, J. Appl. Polym. Sci. 92 (2004) 2163–2168.


[6] D.D. Lu, J.C. Yuan, L.G. You, Z.Q. Lei, Synthesis and NMR characterization of multi- hydroxyl end-groups PEG and PLGA-PEG barbell-like copolymers, J. Macromol. Sci., Part A: Pure Appl. Chem. 45 (2008) 705–711.


[7] H. Peng, Y. Xiao, X. Mao, L. Chen, R. Crawford, A.K. Whittaker, Amphiphilic triblock copolymers of methoxy-poly(ethylene glycol)-b-poly(L-lactide)-b-poly(L-lysine) for enhancement of osteoblast attachment and growth, Biomacromolecules 10 (2009).


[8] S.K. Mehta, N. Jindal, G. Kaur, Quantitative investigation, stability and in vitro release studies of anti-TB drugs in triton niosomes, Colloids Surf. B 87 (2011) 173–179.