Research on the Preparation of Fir Bacterial Fertilizer Using Biological Material


Article Preview

The azotobacter bacteria and the phosphate-solubilizing bacteria are separated and filtered from the rhizosphere soil of the fir plantation and the enzyme activity of azotobacter bacteria, the solubilizing power of phosphate-solubilizing bacteria and the characteristics of PGPR bacteria to produce IAA are measured in this paper. The results show that: there are 5 of the 16 azotobacter bacteria whose enzyme activity is greater than 150 nmol•mL-1•h-1, respectively NGJ-4, NGX-5, NGX-4, NGX-8 and NGJ-8. Py16, Py10 and Py3 own the strongest capacity to dissolve organic phosphorus, respectively 71.31 mg / L, 59.07 mg of / L and 65.14 mg / L; Pw10,Pw6 and Pw20 own the strongest capacity to dissolve inorganic phosphorus, respectively 232.0 mg/L,185.9 mg/L,172.6 mg/L. Py18,Py16 and Py3 own the strongest capacity to produce IAA and dissolve the organic phosphorus bacteria, respectively 38.80mg / L, and 37.29mg / L, and 35.79mg / L; Pw6, Pw8 and Pw21 own the strongest capacity to produce IAA and dissolve the inorganic phosphorus bacteria, respectively 45.340 mg/L, 39.340 mg/L, 27.480 mg/ L. Based on these results, the strains of NGJ-4, NGX-5 and NGJ-8 are selected to prepare the microbial compound bacterial fertilizer using Py16, Py3 and Pw6. Then the azotobacter bacteria, organic phosphate-solubilizing bacteria and inorganic phosphate-solubilizing bacteria is respectively diluted to the solution with the ratio of 15%, and then mix them with the volume proportion of 1.5:1:1 to obtain the mixed bacteria liquid; the proportion of solid carrier ash is 20%, the proportion of fermentation medium is 50%, and the proportion of the mixed bacteria liquid is 30%.



Edited by:

Liangzhong Jiang




D. M. Zhou et al., "Research on the Preparation of Fir Bacterial Fertilizer Using Biological Material", Advanced Materials Research, Vol. 590, pp. 100-105, 2012

Online since:

November 2012




[1] Su Baochuan. Analysis on the Soil Fertility of Pure fir plantation and Mixed Plantation [J]. Anhui Agricultural Science Bulletin, 2007, 13 (1): 138 ~ 140.

[2] Kobus J. The distribution of micro organismsmobilizing pHospHorus in different soils[J]. Acta Microbiology of Polish, 1962, 11: 255~264.

[3] Yao Tuo. Alpine area joint azotobacter bacteria in the rhizosphere of oat II azotobacter bacteria, phosphate solubilization and auxin determination[J]. Grass industry, 2004 (3): 85 ~ 90.

[4] Tian Hong, Zhang Degang, Yao Tuo. A preliminary study on the section of azotobacter bacteria from turfgrass and their partial characterization [J]. Grassland of China, 2005, (5): 47 ~ 52.

[5] Sackett W G, Pattern A G, Brown C W. The solvent action of soil bacteria upon the insoluble pHospHates of raw bone meal and natural raw rock pHospHate[J]. Central Bacterial, 1908, 20: 688~703.

[6] Rmn. Kucey, Hh. Janzen, Me. Legett. Microbially mediated increases in plant-available pHospHorus [J]. Adv. Agron., 1989. 42: 199~228.