Promoting Influence of Organic Carbon Source on Chromate Reduction by Bacillus sp.


Article Preview

The effect of different organic carbon sources on Cr(Ⅵ) reduction by Bacillus sp. was investigated. Three organic compounds, malate, succinate and glucose were tested in Cr(Ⅵ) reduction experiments. The results demonstrated that three organic compounds all significantly enhanced Cr(Ⅵ) reduction. The enhancing degree of these three organic compounds was observed as malate>glucose>succinate, although the cell growth in succinate-added medium was similar to that in malate-added medium and was apparently higher than that in glucose-added medium. Addition of glucose resulted in decrease of pH and increase of redox potential, while addition of malate or succinate resulted in increase of pH and decrease of redox potential during Cr(Ⅵ) reduction. Cr(Ⅵ) was reduced by liquid culture metabolite when glucose was added in liquid medium, but not reduced by liquid culture metabolite when malate or succinate was applied in liquid medium for bacterial cultivation.



Advanced Materials Research (Volumes 610-613)

Edited by:

Qunjie Xu, Yanzhong Ju and Honghua Ge




W. H. Xu et al., "Promoting Influence of Organic Carbon Source on Chromate Reduction by Bacillus sp.", Advanced Materials Research, Vols. 610-613, pp. 1789-1794, 2013

Online since:

December 2012




[1] S. Ozturk, B. Aslim, Z. Suludere, Evaluation of chromium(Ⅵ) removal behaviour by two isolates of Synechocystis sp. in terms of exopolysaccharide (EPS) production and monomer composition, Bioresour. Technol. 100 (2009) 5588-5593.


[2] J. Ye, H. Yin, B. Mai, H. Peng, H. Qin, B. He, N. Zhang, Biosorption of chromium from aqueous solution and electroplating wastewater using mixture of Candida lipolytica and dewatered sewage sludge, Bioresour. Technol. 101 (2010) 3893-3902.


[3] W. Xu, Y. Liu, G. Zeng, X. Li, H. Song, Q. Peng, Characterization of Cr(Ⅵ) resistance and reduction by Pseudomonas aeruginosa, Trans. Nonferrous Met. Soc. China 19 (2009) 1336-1341.


[4] M.N. Kathiravan, R.K. Rani, R. Karthick, K. Muthukumar, Mass transfer studies on the reduction of Cr(Ⅵ) using calcium alginate immobilized Bacillus sp. in packed bed reactor, Bioresour. Technol. 101 (2010) 853-858.


[5] R.C. Patra, S. Malik, M. Beer, M. Megharaj, R. Naidu, Molecular characterization of chromium(Ⅵ) reducing potential in Gram positive bacteria isolated from contaminated sites, Soil Biol. Biochem. 42 (2010) 1857-1863.


[6] C. Desai, K. Jain, D. Madamwar, Evaluation of In vitro Cr(Ⅵ) reduction potential in cytosolic extracts of three indigenous Bacillus sp. isolated from Cr(Ⅵ) polluted industrial landfill, Bioresour. Technol. 99 (2008) 6059-6069.


[7] C. Desai, K. Jain, D. Madamwar, Hexavalent chromate reductase activity in cytosolic fractions of Pseudomonas sp. G1DM21 isolated from Cr(Ⅵ) contaminated industrial landfill, Process Biochem. 43 (2008) 713-721.


[8] A. Pal, S. Dutta, A.K. Paul, Reduction of hexavalent chromium by cell free extract of Bacillus sphaericus AND 303 isolated from serpentine soil, Curr. Microbiol. 51 (2005) 327-330.


[9] Y.G. Liu, W.H. Xu, G.M. Zeng, X. Li, H. Gao, Cr(Ⅵ) reduction by Bacillus sp. isolated from chromium landfill, Process Biochem. 41 (2006) 1981-(1986).


[10] R. Sanghi, A. Srivastava, Long-term chromate reduction by immobilized fungus in continuous column, Chem. Eng. J. 162 (2010) 122-126.


[11] L. Xu, M. Luo, W. Li, X. Wei, K. Xie, L. Liu, C. Jiang, H. Liu, Reduction of hexavalent chromium by Pannonibacter phragmiterus LSSE-09 stimulated with external electron donors under alkaline conditions, J. Hazard. Mater. 185 (2011) 1169-1176.


[12] L. Philip, L. Iyengar, C. Venkobachar, Cr(Ⅵ) reduction by Bacillus coagulans isolated from contaminated soils, J. Environ. Eng. 124 (1998) 1165-1170.


[13] S. Llovera, R. Bonet, M. Simon-Pujol, F. Congregado, Chromate reduction by resting cells of Agrobacterium radiobacter EPS-916, Appl. Environ. Microbiol. 59 (1993) 3516-3518.


[14] H. Shen, Y.T. Wang, Biological reduction of chromium by E. Coli, J. Environ. Eng. 120 (1994) 560-572.

[15] Y-T Wang, C. Xiao, Factors affecting hexavalent chromium reduction in pure cultures of bacteria, Water Res. 29 (1995) 2467-3474.

[16] R.J. Bartlett, J.M. Kimble, Behavior of chromium in soils: II. Hexavalent forms, J. Environ. Qual. 5 (1976) 383-386.

[17] B. Liang, J. Lan, Y.Q. Lan, Comparison of the reduction of Cr(Ⅵ) by mandelic, malic and lactic acids, Acta Sci. Circum. 27(8) (2007) 1326-1330 (In Chinese).

[18] J. Sun, R.N. Dai, Y.Q. Lan, Al3+ catalyzed reduction of Cr(Ⅵ) by tartaric acid, Acta Sci. Circum. 27(8) (2007) 1331-1335 (In Chinese).