Bio-Treatment of Brewery Effluent Using Enzymes


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The feasibility of treating brewery effluent using bio-catalysts (enzymes) was investigated. Carbohydrase, lipase, protease and a mixture enzyme made of carbohydrate and lipase were used. Brewery wastewater samples were collected and analyzed for 96 hrs, at intervals of 12 hrs. The physiochemical properties: biological oxygen demand BOD, chemical oxygen demand COD, total suspended solid (TSS), total dissolved solid TDS, pH, phosphorus (PO4), ammonium nitrogen (NH4-N), total hydrocarbon (THC) were determined using APHA standard method of analysis. The concentrations of the physiochemical properties of the brewery effluent decreased with increasing biodegradation time, for the various enzymes used. However, the different enzymes gave different percentage reductions of each of the physiochemical properties. The order of percentage reduction for the enzyme systems is mixed enzyme > carbohydrase > lipase > protease > control. Hence, the mixed enzyme gave the best results of 90%, 90%, 50%, 100%, 91.6% and 100% reductions for COD, BOD, TSS, NH4-N, PO4 and THC, respectively after 96 hrs. of biotreatment. However, the control gave the least percentage reductions of 6.9%, 5.3%, 16.4%, 7.1%, 5.8% and 50.0% for COD, BOD5, TSS, PO4, NH4-N, and THC respectively.



Advanced Materials Research (Volumes 62-64)

Edited by:

Prof. A.O. Akii Ibhadode, A.I. Igbafe and B.U. Anyata






F.A. Aisien et al., "Bio-Treatment of Brewery Effluent Using Enzymes", Advanced Materials Research, Vols. 62-64, pp. 774-778, 2009

Online since:

February 2009




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