The water cycle is an obvious mode of transmission of enteric diseases. Bacteriologically polluted water is potentially dangerous to health because of possible outbreak of typhoid, dysentery and cholera epidemics. In this paper major sources of drinking water in Ado Ekiti and its environs were examined for physical, chemical and biological qualities. These consists of wells in Adebayo, Ajilosun, Odo-Ado and other suburbs; most popular sachet waters (pure water) produce in Ado- Ekiti and the Pipe-borne water from the state water cooperation (Ureje treatment plants). The results were analyzed using t-distribution method of data analysis and World Health Organization (WHO) Standard was applied. The wells were found to be a little more acidic. All the samples showed high calcium content and some gave total hardness value above WHO standard. 70% of the samples, failed bacteriological test. Samples examined give a wider coverage of drinking water sources in Ado-Ekiti, Ekiti State.