Directing Osteoblast Alignment and Elongation on the Micro-Grooved Silica-Based Hybrid Membrane


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Silica-chitosan hybrid was synthesized from a mixture of chitosan and glycidyloxypropyltrimethoxysilane and then coated on a microgrooved polydimethylsiloxane (PDMS) mold to produce the microgrooved silica-chitosan hybrid membrane. After incubated with osteoblast-like MC3T3-E1 cells, the microgrooved hybrid membranes strongly directed the alignment and elongation of cells and exhibited a strong cell contact guidance ability, indicating a potential application as engineering bone tissue regenerative materials.



Edited by:

Tingting Wang




S. Chen and N. Hanagata, "Directing Osteoblast Alignment and Elongation on the Micro-Grooved Silica-Based Hybrid Membrane", Advanced Materials Research, Vol. 647, pp. 165-169, 2013

Online since:

January 2013




[1] L.L. Hench: J. Mater. Sci: Mater. Med. Vol. 17 (2006), p.967.

[2] L. Hench and J.W. Wilson: Science Vol. 226 (1984), p.630.

[3] T. Kokubo and H. Takadama: Biomaterials Vol. 27 (2006), p.2907.

[4] S. Chen, A. Osaka, S. Hayakawa, K. Tsuru, E. Fujii and K. Kawabata: J. Sol-Gel Sci. Technol. Vol. 48 (2008), p.322.

[5] S. Chen, S. Hayakawa, Y. Shirosaki, E. Fujii, K. Kawabata, K. Tsuru and A. Osaka: J. Am. Ceram. Soc. Vol. 92 (2009), p. (2074).

[6] S. Chen, A. Osaka, S. Hayakawa, Y. Shirosaki and K. Tsuru: J. Mater. Chem. Vol. 20 (2010), p.7337.

[7] S. Chen, A. Osaka, T. Ikoma, H. Morita, J. Li, M. Takeguchi and N. Hanagata: J. Mater. Chem. Vol. 21 (2011), p.10942.

[8] S. Chen, S. Chinnathambi, X.T. Shi, A. Osaka, Y.F. Zhu and N. Hanagata: J. Mater. Chem. Vol. 22 (2012), p.21885.

[9] M. Dash, F. Chiellini, R.M. Ottenbrite and E. Chiellini: Prog. Polym. Sci. Vol. 36 (2011), p.981.

[10] J.K. Suh and H.W. Matthew: Biomaterials Vol. 21 (2000), p.2589.

[11] A. Di Martino, M. Sittinger and M.V. Risbud: Biomaterials Vol. 26 (2005), p.5983.

[12] E.J. Lee, D.S. Shin, H.E. Kim, H.W. Kim, Y.H. Koh and J.H. Jang: Biomaterials Vol. 30 (2009), p.743.

[13] Y. Shirosaki, K. Tsuru, S. Hayakawa, A. Osaka, M.A. Lopes, J.D. Santos and M.H. Fernandes, Biomaterials Vol. 26 (2005), p.485.


[14] H. Aubin, J. W. Nichol, C.B. Hutson, H. Bae, A.L. Sieminski, D.M. Cropek, P. Akhyari and A. Khademhosseini, Biomaterials Vol. 31 (2010), p.6941.


[15] A. Khademhosseini and R. Langer, Biomaterials Vol. 28 (2007), p.5087.

[16] G.A. Dunn and J.P. Heath, Exp. Cell Res. Vol. 101 (1976), p.1.