Erythropoietin Gene Expression in the Stromal Cell Increased by Silica to Induce Erythrocyte Differentiation


Article Preview

Silica containing materials are often applied in bone tissue engineering, which may contact with bone marrow cells. However, the biological effects have not always been observed in studies of bone marrow cells exposed to silica. In this experiment, the relevant biological effects were evaluated. Bone marrow cells and stromal cells treated with silica particles (0.5-10 μm) were applied to investigate the possible interaction mechanism. HEL-92 cells were culture with the condition medium of stromal cells treated with or without silica particles. The erythrogenesis of bone marrow cells treated with silica particles was increased significantly. The expression level of glycophorin A the erythroid marker in HEL-92 cells treated by condition medium was higher than control group. The silica particles could also up-regulate the erythropoietin gene expression of stromal cells. The results indicate that bone marrow cells can be stimulated by silica particles to differentiate into erythrocytes. Our results suggest that silica particles can stimulate bone marrow cells to differentiate erythrocytes possibly via enhancing gene expression of erythropoietin.



Edited by:

Tingting Wang




W. C. Liu et al., "Erythropoietin Gene Expression in the Stromal Cell Increased by Silica to Induce Erythrocyte Differentiation", Advanced Materials Research, Vol. 647, pp. 494-498, 2013

Online since:

January 2013




[1] R. Abdul Jalil, Y. Zhang: Biomaterials Vol 29 (2008), p.4122.

[2] J. Feng, W. Yan, Z. Gou, W. Weng, D. Yang: J Mater Sci Mater Med Vol 18 (2007), p.2167.

[3] J. Korventausta, M. Jokinen, A. Rosling, T. Peltola, A. Yli-Urpo: Biomaterials Vol 24 (2003), p.5173.


[4] Y.G. Figueroa, A.K. Chan, R. Ibrahim, Y. Tang, M.E. Burow, J. Alam, A.B. Scandurro, B.S. Beckman: Exp Hematol Vol 30 (2002), p.1419.

[5] J.M. Carter, K.E. Driscoll: J Environ Pathol Toxicol Oncol Vol 20 (2001), p.33.

[6] D.W. Porter, J. Ye, J. Ma, M. Barger, V.A. Robinson, D. Ramsey, J. McLaurin, A. Khan, D. Landsittel, A. Teass, V. Castranova: Inhal Toxicol Vol 14 (2002), p.349.


[7] X. Shi, Y. Wang, L. Ren, N. Zhao, Y. Gong, D.A. Wang: Acta Biomater Vol 5 (2009), p.1697.

[8] A.J. Mieszawska, L.D. Nadkarni, C.C. Perry, D.L. Kaplan: Chem Mater Vol 22 (2012), p.5780.

[9] W. Piacibello, F. Sanavio, L. Garetto, A. Severino, A. Dane, L. Gammaitoni, M. Aglietta: Leukemia Vol 12 (1998), p.718.


[10] P. Valent, E. Spanblochl, W.R. Sperr, C. Sillaber, K.M. Zsebo, H. Agis, H. Strobl, K. Geissler, P. Bettelheim, K. Lechner: Blood Vol (1992), p.2237.

[11] L.N. Luong, J. Ramaswamy, D.H. Kohn: Biomaterials Vol 33 (2012), p.283.

[12] L.G. Guang, A.L. Boskey, W. Zhu: Int J Biochem Cell Biol Vol 44 (2012), p.1825.

[13] A. Gonzalez, M. Friend, A. Moreno, C.O. Pintado, P. Vogeli, D. Llanes: Anim Genet Vol 26(1995), p.351.